Laser scanning confocal microscope

激光扫描共焦显微镜

• 批准号: 7791586
• 负责人: Bonnie Bartel
• 金额: $ 50万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791586
• 关键词: Area; Arts; Biology; Calcium; Cells; Cellular biology; Chimeric Proteins; Confocal Microscopy; Development; Dyes; Fluorochrome; Funding; Future; Investigation; Lasers; Microscope; Microscopy; Noise; Regulation; Research; Research Personnel; Research Project Grants; Rice; Scanning; Second Messenger Systems; Technology; Tissue Engineering; United States National Institutes of Health; Universities; Work; analytical method; flexibility; improved; protein distribution; second messenger; tool

DESCRIPTION (provided by applicant): Fluorescent microscopy has revolutionized modern biology. From calcium sensitive dyes that track the exquisite regulation of this second messenger to fluorescent fusion proteins that allow us to pinpoint the subcellular distribution of proteins, our ability to observe the inner workings of a cell is constantly expanding. To take advantage of the benefits of these advances we require state-of-the-art microscopy tools. Our current demand for confocal microscopy has exceeded our capacity and our current technology. We are requesting funding for a Zeiss LSM 710 confocal microscope to support our investigation of diverse aspects of developmental and cellular biology with biomedical applications. This microscope was selected as the most capable laser-scanning microscope that can support the research projects of our users, which include biologists with developmental, cellular, and tissue engineering research areas. Moreover, this microscope is modular and expandable to support future applications, including those not yet envisioned. The LSM 710 has incorporated several improvements over previous Zeiss versions, including higher sensitivity, reduced background noise, improved flexibility for new fluorochromes, and integrated analytical methods and is thus ideally suited for the needs of our expanding group of NIH- funded investigators at Rice University.

描述(申请人提供)：荧光显微镜使现代生物学发生了革命性的变化。从跟踪第二信使精细调节的钙敏感染料到使我们能够精确定位蛋白质的亚细胞分布的荧光融合蛋白，我们观察细胞内部工作的能力正在不断扩大。为了利用这些进步的好处，我们需要最先进的显微镜工具。我们目前对共焦显微镜的需求已经超出了我们的能力和我们目前的技术。我们正在为蔡司LSM 710共聚焦显微镜申请资金，以支持我们在生物医学应用中对发育生物学和细胞生物学的各个方面的研究。该显微镜被选为最有能力的激光扫描显微镜，可以支持我们用户的研究项目，其中包括具有发育、细胞和组织工程研究领域的生物学家。此外，这种显微镜是模块化的，可扩展以支持未来的应用，包括那些尚未设想的应用。与蔡司以前的版本相比，LSM 710集成了多项改进，包括更高的灵敏度、更低的背景噪声、更灵活的新荧光显色剂以及集成的分析方法，因此非常适合我们赖斯大学不断扩大的NIH资助研究人员团队的需求。

项目成果

An inventory of peroxisomal proteins and pathways in Drosophila melanogaster.

• DOI: 10.1111/j.1600-0854.2012.01393.x
• 发表时间: 2012-10
• 期刊: Traffic (Copenhagen, Denmark)
• 作者: Faust JE;Verma A;Peng C;McNew JA
• 通讯作者: McNew JA

Plasmonic nanobubbles enhance efficacy and selectivity of chemotherapy against drug-resistant cancer cells.

• DOI: 10.1002/adma.201103550
• 发表时间: 2012-07-24
• 期刊: ADVANCED MATERIALS
• 影响因子: 29.4
• 作者: Lukianova-Hleb, Ekaterina Y.;Ren, Xiaoyang;Zasadzinski, Joseph A.;Wu, Xiangwei;Lapotko, Dmitri O.
• 通讯作者: Lapotko, Dmitri O.

Malaria theranostics using hemozoin-generated vapor nanobubbles.

• DOI: 10.7150/thno.9128
• 发表时间: 2014
• 期刊: Theranostics
• 影响因子: 12.4
• 作者: Lukianova-Hleb EY;Lapotko DO
• 通讯作者: Lapotko DO

Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles.

细胞特异性的跨膜注射分子货物用金纳米颗粒产生的瞬态等离子体纳米泡。

• DOI: 10.1016/j.biomaterials.2012.03.077
• 发表时间: 2012-07
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Lukianova-Hleb, Ekaterina Y.;Wagner, Daniel S.;Brenner, Malcolm K.;Lapotko, Dmitri O.
• 通讯作者: Lapotko, Dmitri O.

A viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes.

• DOI: 10.1007/s11103-014-0223-8
• 发表时间: 2014-09
• 期刊: Plant molecular biology
• 影响因子: 5.1
• 作者: Woodward AW;Fleming WA;Burkhart SE;Ratzel SE;Bjornson M;Bartel B
• 通讯作者: Bartel B