Multiphoton Confocal Microscopy for Biomedical Research

用于生物医学研究的多光子共焦显微镜

• 批准号: 7795484
• 负责人: MADESH MUNISWAMY
• 金额: $ 50万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795484
• 关键词: Acute; Advisory Committees; Aging; Anatomy; Animals; Atherosclerosis; Autoimmunity; Biochemistry; Biomedical Research; Blood Vessels; Bone remodeling; Brain; Cell Communication; Cell physiology; Cellular biology; Chronic; Communicable Diseases; Confocal Microscopy; Data; Development; Discipline; Environment; Equipment; Four-dimensional; Funding; Homing; Image; Immune; Infection; Inflammatory; Injury; Interdisciplinary Study; Kinetics; Life; Lung; Malignant Neoplasms; Microscope; Microscopy; Modeling; Organ; Pathologic Processes; Pathology; Pharmacology; Physiology; Production; Pulmonary Hypertension; Pulmonology; Research; Research Institute; Research Personnel; Research Project Grants; Rheumatology; Services; Supervision; System; Translating; Transplantation; Universities; Vascular Permeabilities; anticancer research; in vivo; insight; instrument; medical schools; multi-photon; novel; progenitor; tool; two-photon

DESCRIPTION (provided by applicant): Multiphoton microscopy is a powerful new tool that facilitates the study of discrete cellular processes within the three-dimensional, natural environment of intact organs in living animals. During the past few years, multi- photon microscopy has revolutionized our view of developmental, homeostatic, and pathologic processes. We propose to establish an intravital multiphoton microscopy system at Temple University School of Medicine through the acquisition of a Carl Zeiss LSM 710 Confocal and Two-Photon Microscope, a service which is currently unavailable on campus. The major users of the proposed instrument will be a group of School of Medicine Investigators drawn from a wide range of disciplines, including Biochemistry, Physiology, Pathology, Pharmacology, Anatomy and Cell Biology, Pulmonary Medicine, Cancer Research Institute and Rheumatology. The need to translate experimental data from culture models into living animals and to expand static information of dynamic cellular processes into four- dimensional kinetic analyses in vivo has greatly augmented the need for intra-vital imaging at Temple. Such research cannot be conducted by equipment currently available. Promising preliminary studies documenting ROS production in the lung, vascular targeting of endothelial progenitors, brain vascular permeability in vivo, and immune cell interactions during inflammatory and infectious diseases has demonstrated the feasibility of multi-photon microscopy for the proposed projects. The instrument will be centrally located in the School of Medicine to enable convenient access for all users; will be maintained by Biochemistry Department under the supervision of the PI and Microscope Advisory Committee. Investigators from diverse fields will have new opportunities for interaction and the initiation of interdisciplinary research projects, which will significantly advance their NIH-funded projects. It is anticipated that the instrument will help to obtain novel insights into the normal cellular physiology, homing of progenitors, and various pathologies involved with aging, cancer, acute organ injury, bone remodeling, transplantation, pulmonary hypertension, atherosclerosis, autoimmunity, and chronic infections.

描述（由申请人提供）：多光子显微镜是一种强大的新工具，有助于研究活体动物完整器官的三维自然环境中的离散细胞过程。在过去的几年里，多光子显微镜已经彻底改变了我们对发育、稳态和病理过程的看法。我们建议通过收购卡尔蔡司LSM 710共聚焦和双光子显微镜，在坦普尔大学医学院建立活体多光子显微镜系统，该服务目前在校园内不可用。拟议仪器的主要用户将是一组来自广泛学科的医学院研究人员，包括生物化学，生理学，病理学，药理学，解剖学和细胞生物学，肺医学，癌症研究所和流变学。需要将来自培养模型的实验数据转化为活体动物，并将动态细胞过程的静态信息扩展为体内四维动力学分析，这极大地增加了对Temple活体成像的需求。这种研究无法通过现有设备进行。有希望的初步研究记录了肺中ROS的产生，内皮祖细胞的血管靶向，体内脑血管通透性，以及炎症和感染性疾病期间的免疫细胞相互作用，这些研究证明了多光子显微镜用于拟议项目的可行性。该仪器将位于医学院的中心位置，以便所有用户方便访问;将由生物化学系在PI和显微镜咨询委员会的监督下进行维护。来自不同领域的研究人员将有新的机会进行互动，并启动跨学科研究项目，这将大大推进他们的NIH资助的项目。预计该仪器将有助于获得对正常细胞生理学、祖细胞归巢以及与衰老、癌症、急性器官损伤、骨重塑、移植、肺动脉高压、动脉粥样硬化、自身免疫和慢性感染有关的各种病理学的新见解。

项目成果

MICU1 motifs define mitochondrial calcium uniporter binding and activity.

• DOI: 10.1016/j.celrep.2013.11.026
• 发表时间: 2013-12-26
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Hoffman NE;Chandramoorthy HC;Shamugapriya S;Zhang X;Rajan S;Mallilankaraman K;Gandhirajan RK;Vagnozzi RJ;Ferrer LM;Sreekrishnanilayam K;Natarajaseenivasan K;Vallem S;Force T;Choi ET;Cheung JY;Madesh M
• 通讯作者: Madesh M

Gamma secretase-activating protein is a substrate for caspase-3: implications for Alzheimer's disease.

• DOI: 10.1016/j.biopsych.2014.06.003
• 发表时间: 2015-04-15
• 期刊: BIOLOGICAL PSYCHIATRY
• 影响因子: 10.6
• 作者: Chu, Jin;Li, Jian-Guo;Joshi, Yash B.;Giannopoulos, Phillip F.;Hoffman, Nicholas E.;Madesh, Muniswamy;Pratico, Domenico
• 通讯作者: Pratico, Domenico

Degradation of gamma secretase activating protein by the ubiquitin-proteasome pathway.

• DOI: 10.1111/jnc.13011
• 发表时间: 2015-05
• 期刊: Journal of neurochemistry
• 影响因子: 4.7
• 作者: Chu J;Li JG;Hoffman NE;Madesh M;Praticò D
• 通讯作者: Praticò D

Visualization of vascular Ca2+ signaling triggered by paracrine derived ROS.

旁分泌衍生的 ROS 触发的血管 Ca2 信号传导的可视化。

• DOI: 10.3791/3511
• 发表时间: 2011
• 期刊: Journal of visualized experiments : JoVE
• 作者: Mallilankaraman,Karthik;Gandhirajan,RajeshKumar;Hawkins,BrianJ;Madesh,Muniswamy
• 通讯作者: Madesh,Muniswamy