Roche 454 Next Generation Sequencer for Human Microbiome and Infectious Disease R

Roche 454 下一代人类微生物组和传染病测序仪 R

• 批准号: 7794216
• 负责人: JANE M CARLTON
• 金额: $ 48.55万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-25 至 2011-03-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794216
• 关键词: Arts; Base Pairing; Communicable Diseases; Communities; Copy Number Polymorphism; Core Facility; DNA Methylation; DNA Resequencing; DNA Sequencing Facility; Data; Disease; Equipment; Funding; Gastroesophageal reflux disease; Gene Expression Profile; Genome; HIV; Hour; Human Microbiome; Individual; Institutes; Intestines; Length; Malaria; Measures; Medical center; New York; Psoriasis; RNA analysis; Reading; Running; Scientist; Services; Small RNA; Technology; Time; Universities; abstracting; cost; instrument; metagenomic sequencing; next generation; pathogen; research study; skin disorder

DESCRIPTION (provided by applicant): ABSTRACT The DNA Sequencing Facility within the Skirball Institute at New York University Langone Medical Center provides sequencing services to about 200 labs and hundreds of scientists in the NYU community. As a core facility, the sequencing lab offers state-of-the-art equipment and expertise, which would be impractical for most individual labs to maintain. We are requesting funds to purchase a Roche Genome Sequencer FLX (GS FLX), developed by 454 Sequencing, a Roche corporation. This pyrosequencing instrument generates over one million unique high quality sequencing reads with an average of 350 to 400 bases pairs in length within a ten hour instrument run time. The total average yield per instrument run is 400 to 500 megabases, and since it is possible to run the instrument multiple times a week, gigabases of useable data can be produced. The instrument supports a wide variety of applications, including de novo pathogen sequencing, metagenomics, target region resequencing, transcriptome sequencing, SNP identification, small RNA analysis, copy number variation, and DNA methylation. The platform would provide the Medical Center with the ability to support ongoing NIH-funded projects at a significantly reduced cost compared to traditional Sanger technologies, with a turn around time that can be measured in days to weeks instead of months to years. Such experiments will greatly increase our understanding of the causes and mechanisms of a number of diseases of the skin, intestinal tract and gastroesophageal tract, such as psoriasis and gastroesophageal reflux disorders, as well as infectious diseases such as malaria and HIV.

描述（由申请人提供）：