Hybrid Organic-Inorganic Composite Materials for Bone Repairs

描述（由申请人提供）：生物玻璃和生物陶瓷在骨修复中有临床应用。然而，由于它们的脆性和较差的力学性能，它们的应用通常仅限于非承重条件。由生物活性无机相（生物玻璃、陶瓷或羟基磷灰石）与高机械性能的生物相容性有机聚合物相剪切混合制成的聚合物复合材料引起了人们的强烈兴趣，因为它与天然骨相似，是磷灰石和胶原基质的复合材料。合成复合材料中有机聚合物相与无机磷灰石或生物玻璃相之间的界面结合较差，导致其作为骨组织工程支架的应力传递能力较低，特别是在承载条件下。为了实现高应力传递，需要对两相进行化学整合。这里提出的具体工作目标是：1。采用溶胶-凝胶技术制备三维大孔杂化材料，将无机生物玻璃（CaO-SiO2-ZnO）与多种有机相结合：a.生物相容性有机聚合物，聚富马酸丙烯和聚三甲氧基硅基甲基丙烯酸丙酯(PTPMA)-与生物玻璃化学集成。通过溶胶-凝胶工艺将硅烷功能化碳纳米管与生物玻璃集成。c.水热技术在边壁功能化碳纳米管（- coo -、HO-和膦酸基）上形成纳米磷灰石的成核。这些合成的复合材料有望在生物玻璃的基础上表现出优异的骨结合能力，并具有更好的力学性能，从而在骨组织工程中得到应用，特别是作为支架。我们还将开发成分-性能关系，为预测制造变量（有机：无机基质的比例，孔隙率，聚合物类型和聚合物与共聚物的比例）与散装生物玻璃相比如何影响生物活性（骨结合能力）和与天然骨相比的机械性能提供指导。生物活性将使用模拟体液和使用人类胎儿成骨细胞进行细胞研究。拟议工作的另一个目的是汇集互补的专业知识，以支持PVAMU强大的生物医学研究，特别是提高各自研究人员的研究效率和学术水平。本研究将制造出具有优异的骨结合能力和改进的机械性能的混合复合材料，而不会出现使用自体骨和同种异体骨修复缺陷或病变骨的问题。本项目涉及合成复合材料的制造，可用于骨修复或骨疾病的治疗。这些轻质复合材料具有生物相容性，能够刺激体液中骨矿物质的形成。

DESCRIPTION (provided by applicant): Bioglass and Bioceramics have found clinical applications in bone repairs. However, because of their brittleness and poor mechanical properties, their applications are generally limited to non load bearing conditions. Polymer composites fabricated by shear mixing of bioactive inorganic phase (bioglass, ceramics, or hydroxyl apatite) with high mechanical performance biocompatible organic polymer phase are eliciting strong interest because of the similarity with natural bone, a composite of apatite and collagen matrix. The poor interfacial bonding between the organic polymer phase and the inorganic apatite or bioglass phase in synthetic composites generally give rise to lower stress transfer ability in applications as scaffolds for bone tissue engineering especially under load bearing condition. To achieve high stress transfer, chemical integration of the two phases would be required. The specific aims of the work proposed here are: 1. To fabricate by sol-gel techniques 3-D macroporous hybrid materials incorporating inorganic bioglass (CaO-SiO2-ZnO) with a variety of organic phases: a.Biocompatible organic polymers , poly(propylene fumarate) and poly(trimethoxysilyl)propylmethacrylate (PTPMA)-chemically integrated with bioglass. b.Silane functionalized carbon nanotubes integrated with bioglass by sol-gel processing. c.Nucleation of nano-apatite on side wall functionalized carbon nanotubes (-COO-, HO- and phosphonic acid groups) using hydrothermal technique. These fabricated composites are expected to show excellent bone bondind ability arising from bioglass with improved mechanical properties and thus find application, especially as scaffolds in bone tissue engineering. We shall also develop composition-property relationship to provide guidance for predicting how fabrication variables, (ratio of organic : inorganic matrix, porosity, type of polymer and polymer to co-polymer ratio) affect bioactivity (bone bonding ability) when compared to bulk bioglasses and mechanical properties compared to natural bone. The bioactivity will be conducted using simulated body fluid and cell studies using human fetal osteoblastic cells. Another aim of the proposed work is to bring together complementary expertise to support strong biomedical research at PVAMU and specifically, enhance research productivity and scholarship of respective investigators. This proposed study will fabricate hybrid composites materials with excellent bone bonding ability and improved mechanical properties without the problems associated with the use of autografts and allografts in repairing defective or diseased bone This project involves the fabrication of synthetic composite materials that can be utilized in bone repair or treatment of bone diseases. These lightweight composites are biocompatible and are able to stimulate bone mineral formation in body fluid.