Hybrid Organic-Inorganic Composite Materials for Bone Repairs

描述(申请人提供)：生物玻璃和生物陶瓷已经在骨修复中得到了临床应用。然而，由于它们的脆性和较差的力学性能，它们的应用通常局限于非承载条件。由生物活性无机相(生物玻璃、陶瓷或羟基磷灰石)与高力学性能的生物相容有机聚合物相剪切混合而成的聚合物复合材料因其与天然骨的相似性而引起人们的极大兴趣。在骨组织工程支架材料中，有机聚合物相与无机磷灰石或生物玻璃相界面结合较差，导致其应力传递能力较差，特别是在承载条件下。为了实现高的应力传递，需要两相的化学整合。本工作的具体目标是：1.利用溶胶-凝胶技术制备含有多种有机相的无机生物玻璃(CaO-SiO_2-ZnO)的三维大孔杂化材料：a.生物相容性有机聚合物、聚(富马酸丙二酯)和聚甲基丙烯酸丙酯(PTPMA)--与生物玻璃化学结合。B.硅烷功能化碳纳米管与生物玻璃通过溶胶-凝胶法集成。C.利用水热技术将纳米磷灰石在侧壁功能化碳纳米管(-COO-、HO-和膦酸基)上成核。这些制备的复合材料有望表现出良好的骨结合能力，提高生物玻璃的力学性能，从而得到应用，特别是作为骨组织工程的支架。我们还将开发成分-性能关系，以提供指导，以预测制造变量(有机/无机基质比例、孔隙率、聚合物类型和聚合物与共聚聚合物比例)与块状生物玻璃相比如何影响生物活性(骨结合能力)，以及与天然骨相比的机械性能。生物活性将使用模拟体液和使用人胎儿成骨细胞的细胞研究进行。拟议工作的另一个目标是汇集互补的专业知识，以支持PVAMU强大的生物医学研究，具体地说，提高研究效率和各自研究人员的学术水平。这项研究将制备具有良好的骨结合能力和改善力学性能的混杂复合材料，而不存在使用自体和同种异体骨修复缺陷或疾病的问题。该项目涉及合成复合材料的制备，可用于骨修复或骨疾病的治疗。这些轻质复合材料具有生物相容性，能够刺激体液中的骨矿物质形成。

DESCRIPTION (provided by applicant): Bioglass and Bioceramics have found clinical applications in bone repairs. However, because of their brittleness and poor mechanical properties, their applications are generally limited to non load bearing conditions. Polymer composites fabricated by shear mixing of bioactive inorganic phase (bioglass, ceramics, or hydroxyl apatite) with high mechanical performance biocompatible organic polymer phase are eliciting strong interest because of the similarity with natural bone, a composite of apatite and collagen matrix. The poor interfacial bonding between the organic polymer phase and the inorganic apatite or bioglass phase in synthetic composites generally give rise to lower stress transfer ability in applications as scaffolds for bone tissue engineering especially under load bearing condition. To achieve high stress transfer, chemical integration of the two phases would be required. The specific aims of the work proposed here are: 1. To fabricate by sol-gel techniques 3-D macroporous hybrid materials incorporating inorganic bioglass (CaO-SiO2-ZnO) with a variety of organic phases: a.Biocompatible organic polymers , poly(propylene fumarate) and poly(trimethoxysilyl)propylmethacrylate (PTPMA)-chemically integrated with bioglass. b.Silane functionalized carbon nanotubes integrated with bioglass by sol-gel processing. c.Nucleation of nano-apatite on side wall functionalized carbon nanotubes (-COO-, HO- and phosphonic acid groups) using hydrothermal technique. These fabricated composites are expected to show excellent bone bondind ability arising from bioglass with improved mechanical properties and thus find application, especially as scaffolds in bone tissue engineering. We shall also develop composition-property relationship to provide guidance for predicting how fabrication variables, (ratio of organic : inorganic matrix, porosity, type of polymer and polymer to co-polymer ratio) affect bioactivity (bone bonding ability) when compared to bulk bioglasses and mechanical properties compared to natural bone. The bioactivity will be conducted using simulated body fluid and cell studies using human fetal osteoblastic cells. Another aim of the proposed work is to bring together complementary expertise to support strong biomedical research at PVAMU and specifically, enhance research productivity and scholarship of respective investigators. This proposed study will fabricate hybrid composites materials with excellent bone bonding ability and improved mechanical properties without the problems associated with the use of autografts and allografts in repairing defective or diseased bone This project involves the fabrication of synthetic composite materials that can be utilized in bone repair or treatment of bone diseases. These lightweight composites are biocompatible and are able to stimulate bone mineral formation in body fluid.