Molecular integration in neuron in vivo

体内神经元的分子整合

• 批准号: 8133430
• 负责人: AKIRA CHIBA
• 金额: $ 33.41万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8133430
• 关键词: Accounting; Bioprobe; Cell Cycle; Cells; Development; Drosophila genus; Energy Transfer; Goals; Grant; Guanosine Triphosphate Phosphohydrolases; In Situ; Individual; Lead; Molecular; Neurons; Property; Resolution; Role; Signal Transduction; Time; base; design; in vivo; nanoscale; receptor; spatiotemporal

DESCRIPTION (provided by applicant): This project develops molecular Bioprobes that can be introduced into Drosophila neurons. These Bioprobes are designed to reveal dynamic signal integrations within an individual neuron in situ. During the initial grant period, the project develops new FRET (fluorescent resonance energy transfer)-based nano-scale Bioprobes to study Cdc42 signaling at the onset of dendrogenesis. The molecular mechanisms of dendrogenesis are not well understood. The complexity among cells and molecules in the CNS requires an approach that supports high-resolution analysis. Recent advances in genetically encoded Bioprobes allow an unprecedented opportunity to launch this new study. Preliminary observations lead to the hypothesis that spatiotemporal integration of Cdc42 activation and effector availability accounts for the precise space-time at which dendrogenesis initiates in neuron in vivo. Therefore, the goal for the initial grant period is two-fold: (1) to examine the role of Cdc42 signaling at the onset of dendrogenesis, and (2) to demonstrates the power of a Bioprobe-assisted in vivo approach. The Bioprobe-assisted analysis is readily applicable to studies on various properties of a neuron and its compartments.

项目描述（申请人提供）：本项目开发可导入果蝇神经元的分子生物探针。 这些生物探针被设计用于原位揭示单个神经元内的动态信号整合。 在最初的资助期间，该项目开发了新的基于FRET（荧光共振能量转移）的纳米级生物探针，以研究树木发生开始时的Cdc42信号。 树木发生的分子机制还不清楚。 CNS中细胞和分子之间的复杂性需要一种支持高分辨率分析的方法。 基因编码生物探针的最新进展为开展这项新研究提供了前所未有的机会。 初步观察导致的假设，时空整合的Cdc42激活和效应器的可用性占精确的时空在体内神经元中的树突状细胞启动。 因此，初始资助期的目标是双重的：（1）检查Cdc42信号在树木发生开始时的作用，以及（2）证明生物探针辅助体内方法的能力。 生物探针辅助分析很容易适用于神经元及其隔室的各种性质的研究。