FUNCTIONAL NEUROANATOMY OF THE BASOLATERAL AMYGDALA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这一年里，我们继续绘制大鼠和灵长类杏仁基底外侧核特定亚区神经元的生理特性图。我们证明了BLA投射神经元的同步放电受相互作用的调节 在抑制性突触电位和固有的膜振荡之间。此外，我们有证据表明，三种固有的膜电流参与了振荡，这些电流可以是正向修饰的神经递质，通过Gs G蛋白耦合，特别是cAMP第二信使级联系统。 我们继续了这方面的研究，现在正在使用体外钙成像技术来研究细胞内钙水平的波动如何影响BLA投射神经元的固有膜振荡。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the year we continued to map the physiological properties of neurons in defined sub-regions of the rat and primate basolateral amygdala. We demonstrated that synchronized firing in BLA projection neuron is regulated by an interaction between inhibitory synaptic potentials and an intrinsic membrane oscillation. Moreover, we have evidence that three intrinsic membrane currents contribute to the oscillation and that these currents can be positively modified neurotransmitters that couple through the Gs G-protein and specifically the cAMP second messenger cascade system. We have continued this line of investigation and are now using in vitro calcium imaging techniques to examine how fluctuations in cytosolic calcium levels may influence the intrinsic membrane oscillations in BLA projection neurons.