GENETIC DIVERSITY AMONG RHESUS ENTERIC CALICIVIRUSES

恒河猴肠杯状病毒的遗传多样性

• 批准号: 8172967
• 负责人: KAROL SESTAK
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172967
• 关键词: Amino Acid Motifs; Animal Model; Blood Group Antigens; Calicivirus; Computer Retrieval of Information on Scientific Projects Database; Diagnostic; Enteral; Epidemiology; Evolution; Feces; Funding; Gastroenteritis; Genetic; Genetic Variation; Grant; Herd Immunity; Human; Immune; Institution; Macaca mulatta; Modeling; Norovirus; Phylogenetic Analysis; Phylogeny; RNA-Directed RNA Polymerase; Reporting; Research; Research Personnel; Resources; Sampling; Serotyping; Source; System; Testing; Time; United States National Institutes of Health; Vaccines; Virus; antigen binding; assay development; pathogen; pressure; prototype; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Noroviruses (NV) are important human pathogens, however NV research has been long time hampered by the lack of an efficient tissue culture or animal model. Recently we reported the discovery of a cultivable calicivirus (Tulane virus; TV). Preliminary results obtained through our ongoing studies show close similarities between TVs and NVs in respect to their phylogeny, epidemiology and histo-blood group antigen (HBGA) binding that make the TV system a promising model for human NV gastroenteritis. In this study 325 randomly selected stool samples collected from TNPRC rhesus macaques were tested for the presence of caliciviruses by using a modified degenerated primer set (P289/P290) targeting conserved amino acid motifs in the calicivirus RNA dependent RNA polymerases (RdRp). Thirty six (11%) of the 325 stools samples tested yielded TV specific sequences, indicating that TVs are endemic in the TNPRC colony. According to phylogenetic analysis, the 36 TV isolates can be classified into two genogroups and at least four genetic types. Interestingly, none of the strains were identical to the prototype TV that was isolated from the same colony in 2004. Such wide genetic diversity among rhesus enteric caliciviruses indicates that the epidemiology and evolution of TVs in the TNPRC colony are similar to those of human NVs, possibly associated with wide HBGA repertoire of the host and immune pressure (herd immunity). Thus, rhesus enteric caliciviruses could be used as a model for the evolution and epidemiology of human NVs. The discovery of TV genetic diversity with the tissue culture adaptability of these viruses is important because it opens the avenue for studying the relationship between genetic variation and serotypes (antigenic types) with applications to NV vaccine and diagnostic assay development.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 北病毒（NV）是重要的人类病原体，但是由于缺乏有效的组织培养或动物模型，NV研究已受到困扰。最近，我们报道了发现可栽培的彩色病毒（Tulane病毒；电视）的发现。通过我们正在进行的研究获得的初步结果表明，在其系统发育，流行病学和组织血管群抗原（HBGA）结合方面，电视和NV之间的相似之处是使电视系统成为人类NV胃肠炎的有前途模型。在这项研究中，325个从TNPRC恒河猕猴中收集的随机选择的粪便样品通过使用修饰的退化引物组（P289/P290）靶向保守的氨基酸图像中的钙病毒RNA依赖性RNA RNA聚合酶（RDRP），测试了孔西病毒的存在。在经过测试的325个凳子样品中，有36个（11％）产生了特定的电视序列，表明电视在TNPRC菌落中是特有的。根据系统发育分析，可以将36个电视分离株分为两种基因组和至少四种遗传类型。有趣的是，这些菌株均与2004年从同一菌落中分离出来的原型电视相同。恒河猴肠胃病毒中如此广泛的遗传多样性表明，TNPRC菌落中电视的流行病学和演变与人类NV的相似，可能与人类NV的相似，可能与HBGA eCortertoire和免疫压力相关（她的免疫力和免疫压力）（她的免疫力较大）（她的免疫压力）（她的免疫压力）（她的免疫压力）。因此，恒河猴可以用作人类NVS进化和流行病学的模型。通过这些病毒的组织培养适应能力发现电视遗传多样性很重要，因为它为研究遗传变异与血清型（抗原类型）（抗原类型）之间的关系开辟了途径，并应用于NV疫苗和诊断测定法。