CHARACTERIZATION OF A RHESUS CALICIVIRUS REPRESENTS A NEW GENUS OF CALICIVIRIDAE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 我们报道了一种新的杯状病毒（CV）的特征，它是从杜兰国家灵长类动物研究中心（TNPRC）圈养的幼年恒河猴（Macaca mulatta）的粪便样本中分离出来的。Tulane病毒（TV）的全基因组包含6714个核苷酸（nt）和一个poly-A尾，并被组织成3个开放阅读框（ORF），其编码非结构（NS）多聚蛋白（ORF 1）、估计分子量（Mr）为57.9 kDa的衣壳蛋白（ORF 2）、和一个可能的次要结构蛋白（ORF 3），等电点（pI）为10.0，计算的Mr为22.8 kDa。NS-多聚蛋白显示了所有典型的CV氨基酸基序，包括GXXGXGKT（NTG）、EYXEX（Vpg）、GDCG（蛋白酶）、GLPSG和YGDD（聚合酶）。构建的NS-多聚蛋白，NTR，蛋白酶，聚合酶，和衣壳蛋白序列的系统发育树一致地将电视的分支植根于诺如病毒，但与其他属之间的距离相等。TV可在猴肾细胞系（LLC-MK 2）中培养，出现典型的细胞病变效应（CPE）。TV具有典型的CV形态，直径为36 nm，浮力密度为1.37 g/ml。根据这些理化和遗传特征，TV代表了一个新的CV属，我们建议将其命名为“猴病毒”（Rhesus Enteric CV）。尽管TV在恒河猴中的致病性仍有待阐明，但TV引起肠道感染的可能性和组织培养系统的可用性使得这种病毒成为人类CV的有价值的替代品。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We reported the characterization of a novel calicivirus (CV) that was isolated from stool samples of captive juvenile rhesus macaques (Macaca mulatta) of the Tulane National Primate Research Center (TNPRC). The complete genome of the Tulane virus (TV) contains 6714 nucleotides (nt) plus a poly-A tail and is organized into 3 open reading frames (ORF) which encode the nonstructural (NS) polyprotein (ORF1), the capsid protein (ORF2) with an estimated molecular mass (Mr) of 57.9 kDa, and a possible minor structural protein (ORF3) with a isoelectric point (pI) of 10.0 and calculated Mr of 22.8 kDa. The NS- polyprotein revealed all typical CV amino acid motifs, including GXXGXGKT (NTPase), EYXEX (Vpg), GDCG (protease), GLPSG and YGDD (polymerase). Phylogenetic trees constructed for the NS-polyprotein, NTPase, protease, polymerase, and capsid protein sequences consistently placed the TV on a branch rooted with Norovirus but with distances equal to those between other genera. The TV can be cultured in a monkey kidney cell line (LLC-MK2) with the appearance of typical cytopathic effect (CPE). TV exhibits a typical CV morphology with a diameter of 36 nm and has a buoyant density of 1.37 g/ml. According to these physicochemical and genetic characteristics, TV represents a new CV genus for which we propose the name "Recovirus" (Rhesus Enteric CV). Although the pathogenicity of TV in rhesus macaques remains to be elucidated, the likelihood of TV causing intestinal infection and the availability of a tissue culture system makes this virus a valuable surrogate to human CVs.