CHARACTERIZATION OF A RHESUS CALICIVIRUS REPRESENTS A NEW GENUS OF CALICIVIRIDAE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本文报道了从杜兰国家灵长类动物研究中心(TNPRC)圈养幼年恒河猴(Macaca Mulatta)粪便标本中分离到的一种新型杯状病毒的特征。杜兰病毒(TV)全基因组由6714个核苷酸(NT)和一个Poly-A尾巴组成，由3个开放阅读框(ORF)组成，分别编码非结构(NS)多蛋白(ORF1)、衣壳蛋白(ORF2)和可能的次要结构蛋白(ORF3)，其等电点(PI)为10.0，等电点(MR)为22.8 kDa。NS-多聚蛋白显示了所有典型的CV氨基酸序列，包括GXXGXGKT(NTPase)、EYXEX(VPG)、GDCG(蛋白酶)、GLPSG和YGDD(聚合酶)。根据NS-多蛋白、NTPase、蛋白酶、聚合酶和衣壳蛋白序列构建的系统发育树一致地将TV置于以诺如病毒为根的分支上，但与其他属之间的距离相同。在猴肾细胞系(LLC-MK2)中可培养出典型的细胞病变效应(CPE)。TV呈典型的CV形态，直径为36 nm，浮力密度为1.37g/ml。根据这些理化和遗传特征，TV代表了一个新的CV属，我们将其命名为新的CV属，我们将其命名为Rheus Enteric CV。虽然TV对恒河猴的致病性仍有待阐明，但TV引起肠道感染的可能性以及组织培养系统的可获得性使该病毒成为人类CV的有价值的替代品。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We reported the characterization of a novel calicivirus (CV) that was isolated from stool samples of captive juvenile rhesus macaques (Macaca mulatta) of the Tulane National Primate Research Center (TNPRC). The complete genome of the Tulane virus (TV) contains 6714 nucleotides (nt) plus a poly-A tail and is organized into 3 open reading frames (ORF) which encode the nonstructural (NS) polyprotein (ORF1), the capsid protein (ORF2) with an estimated molecular mass (Mr) of 57.9 kDa, and a possible minor structural protein (ORF3) with a isoelectric point (pI) of 10.0 and calculated Mr of 22.8 kDa. The NS- polyprotein revealed all typical CV amino acid motifs, including GXXGXGKT (NTPase), EYXEX (Vpg), GDCG (protease), GLPSG and YGDD (polymerase). Phylogenetic trees constructed for the NS-polyprotein, NTPase, protease, polymerase, and capsid protein sequences consistently placed the TV on a branch rooted with Norovirus but with distances equal to those between other genera. The TV can be cultured in a monkey kidney cell line (LLC-MK2) with the appearance of typical cytopathic effect (CPE). TV exhibits a typical CV morphology with a diameter of 36 nm and has a buoyant density of 1.37 g/ml. According to these physicochemical and genetic characteristics, TV represents a new CV genus for which we propose the name "Recovirus" (Rhesus Enteric CV). Although the pathogenicity of TV in rhesus macaques remains to be elucidated, the likelihood of TV causing intestinal infection and the availability of a tissue culture system makes this virus a valuable surrogate to human CVs.