GENETIC DIVERSITY AMONG RHESUS ENTERIC CALICIVIRUSES
恒河猴肠杯状病毒的遗传多样性
基本信息
- 批准号:8358072
- 负责人:
- 金额:$ 3.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-05-01 至 2012-04-30
- 项目状态:已结题
- 来源:
- 关键词:Amino Acid MotifsAnimal ModelBlood Group AntigensCalicivirusDiagnosticEnteralEpidemiologyEvolutionFecesFundingGastroenteritisGeneticGenetic VariationGrantHerd ImmunityHumanImmuneMacaca mulattaModelingNational Center for Research ResourcesNorovirusPhylogenetic AnalysisPhylogenyPrimatesPrincipal InvestigatorRNA-Directed RNA PolymeraseReportingResearchResearch InfrastructureResourcesSamplingSerotypingSourceSystemTestingTimeUnited States National Institutes of HealthVaccinesVirusantigen bindingassay developmentcostpathogenpressureprototypetissue culture
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Noroviruses (NV) are important human pathogens, however NV research has been long time hampered by the lack of an efficient tissue culture or animal model. Recently we reported the discovery of a cultivable calicivirus (Tulane virus; TV). Preliminary results obtained through our ongoing studies show close similarities between TVs and NVs in respect to their phylogeny, epidemiology and histo-blood group antigen (HBGA) binding that make the TV system a promising model for human NV gastroenteritis. In this study 325 randomly selected stool samples collected from TNPRC rhesus macaques were tested for the presence of caliciviruses by using a modified degenerated primer set (P289/P290) targeting conserved amino acid motifs in the calicivirus RNA dependent RNA polymerases (RdRp). Thirty six (11%) of the 325 stools samples tested yielded TV specific sequences, indicating that TVs are endemic in the TNPRC colony. According to phylogenetic analysis, the 36 TV isolates can be classified into two genogroups and at least four genetic types. Interestingly, none of the strains were identical to the prototype TV that was isolated from the same colony in 2004. Such wide genetic diversity among rhesus enteric caliciviruses indicates that the epidemiology and evolution of TVs in the TNPRC colony are similar to those of human NVs, possibly associated with wide HBGA repertoire of the host and immune pressure (herd immunity). Thus, rhesus enteric caliciviruses could be used as a model for the evolution and epidemiology of human NVs. The discovery of TV genetic diversity with the tissue culture adaptability of these viruses is important because it opens the avenue for studying the relationship between genetic variation and serotypes (antigenic types) with applications to NV vaccine and diagnostic assay development.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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KAROL SESTAK其他文献
KAROL SESTAK的其他文献
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{{ truncateString('KAROL SESTAK', 18)}}的其他基金
DEVELOPMENT OF Q PCR ASSAY FOR DETECTION OF ENTERIC CALICIVIRUSES
用于检测肠道杯状病毒的 Q PCR 检测方法的开发
- 批准号:
8358112 - 财政年份:2011
- 资助金额:
$ 3.72万 - 项目类别:
XENOBIOTIC METABOLISM AND CANCER IN GLUTEN-SENSITIVE MACAQUES
麸质敏感猕猴的异生代谢与癌症
- 批准号:
8358154 - 财政年份:2011
- 资助金额:
$ 3.72万 - 项目类别:
ROLE OF RHESUS ROTAVIRUS GENE 4 IN BILIARY ATRESIA
恒河猴轮状病毒基因 4 在胆道闭锁中的作用
- 批准号:
8358153 - 财政年份:2011
- 资助金额:
$ 3.72万 - 项目类别:
CHARACTERIZATION OF GLUTEN-SENSISTIVE RHESUS MACAQUES
麸质敏感恒河猴的特征
- 批准号:
8358073 - 财政年份:2011
- 资助金额:
$ 3.72万 - 项目类别:
NORO-, SAPO- & RHESUS ENTERIC CALICIVIRUS-SPECIFIC ANTIBODIES IN MACAQUES
NORO-、SAPO-
- 批准号:
8358093 - 财政年份:2011
- 资助金额:
$ 3.72万 - 项目类别:
DEVELOPMENT OF RT PCR ASSAY FOR QUANTITATIVE DETECTION OF ENTERIC CALICIVIRUSES
肠道杯状病毒定量检测 RT PCR 检测方法的开发
- 批准号:
8173022 - 财政年份:2010
- 资助金额:
$ 3.72万 - 项目类别:
GENETIC DIVERSITY AMONG RHESUS ENTERIC CALICIVIRUSES
恒河猴肠杯状病毒的遗传多样性
- 批准号:
8172967 - 财政年份:2010
- 资助金额:
$ 3.72万 - 项目类别:
NONINFLAMMATORY GLUTEN PEPTIDE ANALOGUES AS BIOMARKERS FOR CELIAC SPRUE
非炎症性麸质肽类似物作为乳糜泻的生物标志物
- 批准号:
8172996 - 财政年份:2010
- 资助金额:
$ 3.72万 - 项目类别:
NORO-, SAPO- & RHESUS ENTERIC CALICIVIRUS-SPECIFIC ANTIBODIES IN YOUNG MACAQUES
NORO-、SAPO-
- 批准号:
8172995 - 财政年份:2010
- 资助金额:
$ 3.72万 - 项目类别:
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