NORO-, SAPO- & RHESUS ENTERIC CALICIVIRUS-SPECIFIC ANTIBODIES IN YOUNG MACAQUES

NORO-、SAPO-

• 批准号: 8172995
• 负责人: KAROL SESTAK
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172995
• 关键词: Antibodies; B-Lymphocyte Epitopes; Binding; Biological Preservation; Calicivirus; Computer Retrieval of Information on Scientific Projects Database; Data; Disease Outbreaks; Enteral; Enzyme-Linked Immunosorbent Assay; Funding; Grant; High Prevalence; Human; Institution; Macaca; Macaca mulatta; Norovirus; Prevalence; Primates; Research; Research Personnel; Resources; Sampling; Sapovirus; Serum; Source; United States National Institutes of Health; Virus; cross reactivity; neutralizing antibody; prototype; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study was to determine the prevalence of anti-norovirus (NoV), sapovirus (SaV) and Tulane virus (TV) antibodies in rhesus macaques of the Tulane National Primate Research Center (TNPRC) and evaluate the antigenic relationship between these viruses. A high prevalence of NoV (51-61%) and SaV (50-56%) binding antibodies and TV (69%) neutralizing antibodies were detected. Serum samples obtained during a human NoV outbreak and a multivalent anti-NoV hyperimmune serum were not able to neutralize TV infectivity. Conversely, low levels of cross-reactivity between the prototype TV and NoVs, but not between the TV and SaVs were detected by ELISA. These data indicate the preservation of some cross-reactive B-cell epitopes between the rhesus and human CVs. The high prevalence of human and rhesus CV-specific serum antibodies suggests the frequent exposure of colony macaques to enteric CVs including the possibility of CV transmission between human and NHP hosts.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究旨在了解杜兰国家灵长类动物研究中心(TNPRC)猕猴体内抗诺如病毒(NOV)、萨普病毒(SAV)和杜兰病毒(TV)抗体的流行情况，并评价这些病毒之间的抗原性关系。NOV(51-61%)、SAV(50-56%)结合抗体和TV(69%)中和抗体阳性率较高。在一次人类新城疫暴发期间获得的血清样本和一种多价抗新城疫高免血清无法中和TV的传染性。相反，通过酶联免疫吸附试验检测到原型TV和NoV之间的低水平交叉反应，但TV和SAV之间没有检测到交叉反应。这些数据表明，恒河猴和人类CV之间保留了一些交叉反应的B细胞表位。人类和恒河猴CV特异性血清抗体的高流行率表明，群体猕猴经常接触肠道CV，包括在人类和NHP宿主之间传播CV的可能性。