NORO-, SAPO- & RHESUS ENTERIC CALICIVIRUS-SPECIFIC ANTIBODIES IN MACAQUES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本研究旨在了解杜兰国家灵长类动物研究中心(TNPRC)猕猴体内抗诺如病毒(NOV)、萨普病毒(SAV)和杜兰病毒(TV)抗体的流行情况，并评价这些病毒之间的抗原性关系。NOV(51-61%)、SAV(50-56%)结合抗体和TV(69%)中和抗体阳性率较高。在一次人类新城疫暴发期间获得的血清样本和一种多价抗新城疫高免血清无法中和TV的传染性。相反，通过酶联免疫吸附试验检测到原型TV和NoV之间的低水平交叉反应，但TV和SAV之间没有检测到交叉反应。这些数据表明，恒河猴和人类CV之间保留了一些交叉反应的B细胞表位。人类和恒河猴CV特异性血清抗体的高流行率表明，群体猕猴经常接触肠道CV，包括在人类和NHP宿主之间传播CV的可能性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this study was to determine the prevalence of anti-norovirus (NoV), sapovirus (SaV) and Tulane virus (TV) antibodies in rhesus macaques of the Tulane National Primate Research Center (TNPRC) and evaluate the antigenic relationship between these viruses. A high prevalence of NoV (51-61%) and SaV (50-56%) binding antibodies and TV (69%) neutralizing antibodies were detected. Serum samples obtained during a human NoV outbreak and a multivalent anti-NoV hyperimmune serum were not able to neutralize TV infectivity. Conversely, low levels of cross-reactivity between the prototype TV and NoVs, but not between the TV and SaVs were detected by ELISA. These data indicate the preservation of some cross-reactive B-cell epitopes between the rhesus and human CVs. The high prevalence of human and rhesus CV-specific serum antibodies suggests the frequent exposure of colony macaques to enteric CVs including the possibility of CV transmission between human and NHP hosts.