NORO-, SAPO- & RHESUS ENTERIC CALICIVIRUS-SPECIFIC ANTIBODIES IN MACAQUES

NORO-、SAPO-

• 批准号: 8358093
• 负责人: KAROL SESTAK
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358093
• 关键词: Antibodies; B-Lymphocyte Epitopes; Binding; Biological Preservation; Calicivirus; Data; Disease Outbreaks; Enteral; Enzyme-Linked Immunosorbent Assay; Funding; Grant; High Prevalence; Human; Macaca; Macaca mulatta; National Center for Research Resources; Norovirus; Prevalence; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Sapovirus; Serum; Source; United States National Institutes of Health; Virus; cost; cross reactivity; neutralizing antibody; prototype; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this study was to determine the prevalence of anti-norovirus (NoV), sapovirus (SaV) and Tulane virus (TV) antibodies in rhesus macaques of the Tulane National Primate Research Center (TNPRC) and evaluate the antigenic relationship between these viruses. A high prevalence of NoV (51-61%) and SaV (50-56%) binding antibodies and TV (69%) neutralizing antibodies were detected. Serum samples obtained during a human NoV outbreak and a multivalent anti-NoV hyperimmune serum were not able to neutralize TV infectivity. Conversely, low levels of cross-reactivity between the prototype TV and NoVs, but not between the TV and SaVs were detected by ELISA. These data indicate the preservation of some cross-reactive B-cell epitopes between the rhesus and human CVs. The high prevalence of human and rhesus CV-specific serum antibodies suggests the frequent exposure of colony macaques to enteric CVs including the possibility of CV transmission between human and NHP hosts.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本研究的目的是确定抗诺如病毒（NoV），沙波病毒（SaV）和杜兰病毒（TV）抗体在杜兰国家灵长类动物研究中心（TNPRC）的恒河猴中的流行率，并评估这些病毒之间的抗原关系。检测到高流行率的NoV（51-61%）和SaV（50-56%）结合抗体和TV（69%）中和抗体。在人类NoV爆发期间获得的血清样品和多价抗NoV超免疫血清不能中和TV传染性。相反，通过ELISA检测到原型TV和NoV之间的低水平交叉反应性，但TV和SaV之间没有。这些数据表明恒河猴和人CV之间保留了一些交叉反应性B细胞表位。人和恒河猴CV特异性血清抗体的高流行率表明群体猕猴频繁暴露于肠道CV，包括人和NHP宿主之间CV传播的可能性。