ROTAVIRUSES HAVE DIVERGENT GENE CONSTELLATIONS

轮状病毒具有不同的基因群

• 批准号: 8358125
• 负责人: KAROL SESTAK
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358125
• 关键词: Animals; Basic Science; Canis familiaris; Capsid Proteins; Cattle; Cercopithecus tantalus; Data; Family Felidae; Family suidae; Funding; Genes; Genetic; Genome; Genotype; Grant; Human; Macaca; Macaca mulatta; National Center for Research Resources; Phylogenetic Analysis; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Rotavirus; Ruminants; Source; Tail; United States National Institutes of Health; Virus; cost; genome sequencing; progenitor; research and development; transmission process; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Although few simian rotaviruses (RVs) have been isolated, such strains have been important for basic research and vaccine development. To explore the origins of simian RVs, the complete genome sequences of strains PTRV (G8P[1]), RRV (G3P[3]), and TUCH (G3P[24]) were determined. This data allowed the genotype constellations of each virus to be determined and the phylogenetic relationships of the simian strains with each other and with non-simian RVs to be elucidated. The results indicate that PTRV was likely transmitted from a bovine or other ruminant into its host of origin (pig-tailed macaques), as its genes have genotypes and encode outer-capsid proteins similar to those of bovine RVs. In contrast, most of the genes of rhesus-macaque strains, RRV and TUCH, have genotypes more typical of canine-feline RVs. However, the sequences of the canine/feline-like genes of RRV and TUCH are only distantly-related to those of modern canine/feline RVs, indicating that transmission of the progenitor virus from a canine/feline host to a simian host was not recent. The remaining genes of RRV and TUCH appear to have originated through reassortment with bovine, human, or other RV strains. Finally, comparison of PTRV, RRV, and TUCH genes with those of the vervet-monkey RV SA11-H96 (G3P[2]) indicates that SA11-H96 shares little genetic similarity to other simian strains and likely has evolved independently. Collectively, our data indicate that simian RVs are of diverse ancestry with genome constellations that originated largely by interspecies transmission and reassortment with non-human animal RVs.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 虽然很少有猴轮状病毒（RV）已被分离，这些菌株已为基础研究和疫苗开发的重要。 为了探索猿RV的起源，测定了PTRV（G8 P [1]）、RRV（G3 P [3]）和TUCH（G3 P [24]）株的全基因组序列。这些数据使每种病毒的基因型星座得以确定，并阐明了猴病毒株之间以及与非猴病毒株之间的系统发育关系。结果表明，PTRV可能是从牛或其他反刍动物传播到其宿主的起源（猪尾猕猴），因为它的基因有基因型和编码的外衣壳蛋白类似的牛RV。 相比之下，大多数基因的恒河猴株，RRV和TUCH，具有更典型的犬猫RV的基因型。 然而，RRV和TUCH的犬/猫样基因的序列与现代犬/猫RV的序列仅远相关，这表明祖病毒从犬/猫宿主传播到猿宿主不是最近的事。 RRV和TUCH的其余基因似乎起源于与牛、人或其他RV毒株的重配。最后，将PTRV、RRV和TUCH基因与黑猴RV SA 11-H96（G3 P [2]）的基因进行比较，表明SA 11-H96与其他猿猴毒株几乎没有遗传相似性，可能是独立进化的。 总的来说，我们的数据表明，猿类RV是不同的祖先与基因组星座，起源主要是种间传播和重组与非人类动物RV。