ROTAVIRUSES HAVE DIVERGENT GENE CONSTELLATIONS

轮状病毒具有不同的基因群

• 批准号: 8358125
• 负责人: KAROL SESTAK
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358125
• 关键词: Animals; Basic Science; Canis familiaris; Capsid Proteins; Cattle; Cercopithecus tantalus; Data; Family Felidae; Family suidae; Funding; Genes; Genetic; Genome; Genotype; Grant; Human; Macaca; Macaca mulatta; National Center for Research Resources; Phylogenetic Analysis; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Rotavirus; Ruminants; Source; Tail; United States National Institutes of Health; Virus; cost; genome sequencing; progenitor; research and development; transmission process; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Although few simian rotaviruses (RVs) have been isolated, such strains have been important for basic research and vaccine development. To explore the origins of simian RVs, the complete genome sequences of strains PTRV (G8P[1]), RRV (G3P[3]), and TUCH (G3P[24]) were determined. This data allowed the genotype constellations of each virus to be determined and the phylogenetic relationships of the simian strains with each other and with non-simian RVs to be elucidated. The results indicate that PTRV was likely transmitted from a bovine or other ruminant into its host of origin (pig-tailed macaques), as its genes have genotypes and encode outer-capsid proteins similar to those of bovine RVs. In contrast, most of the genes of rhesus-macaque strains, RRV and TUCH, have genotypes more typical of canine-feline RVs. However, the sequences of the canine/feline-like genes of RRV and TUCH are only distantly-related to those of modern canine/feline RVs, indicating that transmission of the progenitor virus from a canine/feline host to a simian host was not recent. The remaining genes of RRV and TUCH appear to have originated through reassortment with bovine, human, or other RV strains. Finally, comparison of PTRV, RRV, and TUCH genes with those of the vervet-monkey RV SA11-H96 (G3P[2]) indicates that SA11-H96 shares little genetic similarity to other simian strains and likely has evolved independently. Collectively, our data indicate that simian RVs are of diverse ancestry with genome constellations that originated largely by interspecies transmission and reassortment with non-human animal RVs.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 尽管很少分离出邻胺轮状病毒（RV），但这种菌株对于基础研究和疫苗开发很重要。 为了探索SINIAN RVS的起源，确定了菌株PTRV（G8P [1]），RRV（G3P [3]）和TUCH（G3P [24]）的完整基因组序列。这些数据允许确定每种病毒的基因型星座以及彼此之间的系统发育关系，并阐明非西米亚RV。结果表明，由于其基因具有基因型并编码类似于牛RV的基因，因此PTRV可能是从牛或其他反刍动物传播到其起源（猪尾猕猴）的宿主（猪尾猕猴）。 相比之下，恒河-晶状体菌株，RRV和TUCH的大多数基因具有更典型的犬 - 五线RV的基因型。 但是，RRV和TUCH的犬/猫样基因的序列仅与现代犬/猫RV的序列相关，这表明祖细胞病毒从犬/猫宿主传播到示例宿主并不是最近的。 RRV和TUCH的其余基因似乎是通过与牛，人或其他RV菌株的重新分类来起源的。最后，PTRV，RRV和TUCH基因与Vervet-Monkey RV SA11-H96（G3P [2]）的比较表明，SA11-H96与其他与其他Simian菌株的遗传相似性几乎没有遗传相似性，并且可能已经独立发展。 总的来说，我们的数据表明，拟南芥的祖先是基因组星座的多样性，主要是由于种间传播和与非人类动物RV的重新分配。