GENETIC ANALYSIS OF GERM CELL FORMATION

生殖细胞形成的遗传分析

• 批准号: 8173332
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173332
• 关键词: Cells; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Germ Cells; Grant; Implant; In Vitro; Institution; Left; Measures; Meiosis; Monkeys; Population; Research; Research Design; Research Personnel; Resources; Seminiferous tubule structure; Series; Source; Spermatogenesis; Testis; Testosterone; Transplantation; United States National Institutes of Health; embryonic stem cell; genetic analysis; human embryonic stem cell; model development; nonhuman primate; stem cell population

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study is to measure germ cell development of the ES cells and their ability to complete meiosis within the seminiferous tubules after transplantation into nonhuman primate testis. Specifically we will first conduct a series of model development studies designed to establish the feasibility of down-regulating spermatogenesis in monkeys with testosterone implants and eliminating spermatogonial stem cell populations with orally administered bisulfan exposure. Next, the left testis will receive populations of human ES cells that have not been subjected to in vitro differentiation while the right testis will receive cells exposed to 14 days of differentiation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是测量胚胎干细胞移植到非人灵长类动物睾丸后的生殖细胞发育和它们在曲细精管内完成减数分裂的能力。 具体而言，我们将首先进行一系列模型开发研究，旨在确定下调睾丸激素植入猴子精子发生的可行性，并消除口服给药的联磺酰暴露的精原干细胞群。 接下来，左侧睾丸将接受未进行体外分化的人ES细胞群，而右侧睾丸将接受暴露于14天分化的细胞。