HISTOCOMPATIBLE PRIMATE EMBYONIC STEM CELLS (ESCS)

组织相容性灵长类胚胎干细胞 (ESCS)

• 批准号: 8357828
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 7.28万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357828
• 关键词: Biological Assay; Cells; Chimera organism; Development; Drug or chemical Tissue Distribution; Embryo; Embryo Transfer; Epigenetic Process; Fetus; Funding; Genetic; Germ Lines; Goals; Grant; In Vitro; Macaca mulatta; Molecular Profiling; Monkeys; Mus; National Center for Research Resources; Pregnancy; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Somatic Cell; Source; Stem cells; Testing; United States National Institutes of Health; blastocyst; comparative; cost; in vivo; induced pluripotent stem cell; insight; offspring; pluripotency; somatic cell nuclear transfer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to generate important new insights concerning reprogramming of primate somatic cells to the pluripotent state employing somatic cell nuclear transfer (SCNT) and direct reprogramming (iPS) approaches and to conduct comparative pluripotency assessments using expression profiling, genetic and epigenetic analysis and in vitro and in vivo differentiation assays. We will also determine the potential of monkey pluripotent cells to contribute to chimeras. We hypothesize that similar to their mouse counterparts, primate ESCs and iPS cells have the potential to integrate and participate in development of chimeric offspring. To test this hypothesis, we propose to inject GFP-expressing monkey ESCs and iPS cells into monkey preimplantation embryos and transfer the resultant chimeric embryos into recipients to establish pregnancies. Chimeric fetuses and full-term offspring will subsequently be studied for tissue distribution and germ line colonization. To date, we carried out a comprehensive expression and epigenetic profiling of rhesus monkey SCNT -ESCs and iPS cells derived from genetically identical parental cells.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目的目标是产生重要的新的见解灵长类动物体细胞的重编程到多能状态，采用体细胞核移植（SCNT）和直接重编程（iPS）的方法，并进行比较多能性评估使用表达谱，遗传和表观遗传分析和体外和体内分化测定。我们还将确定猴子多能细胞促成嵌合体的潜力。我们假设，类似于他们的小鼠同行，灵长类胚胎干细胞和iPS细胞有潜力整合和参与嵌合后代的发展。为了验证这一假设，我们建议将表达GFP的猴胚胎干细胞和iPS细胞注射到猴植入前胚胎中，并将所得的嵌合胚胎移植到受体中以建立妊娠。随后将研究嵌合胎儿和足月后代的组织分布和生殖系定殖。 迄今为止，我们对恒河猴SCNT-ESC和来源于遗传上相同的亲本细胞的iPS细胞进行了全面的表达和表观遗传学分析。