ALTERED NUCLEAR TRANSFER

改变的核转移

• 批准号: 8357769
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 14.55万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357769
• 关键词: Affect; Bypass; CDX2 gene; Cell Lineage; Down-Regulation; Embryo; Funding; Goals; Grant; National Center for Research Resources; Oocytes; Pluripotent Stem Cells; Primates; Principal Investigator; Process; Production; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; egg; embryonic stem cell; nuclear transfer; somatic cell nuclear transfer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to utilize altered somatic cell nuclear transfer into primate eggs with the hope of yielding pluripotent stem cells while bypassing the production/destruction of viable embryos in the process. We showed that the trophectodermal lineage in primate embryos can be abrogated by downregulation of CDX2 in unfertilized oocytes. The pluripotent inner cell lineage was not affected and produced functional ES cells.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 该项目的目标是利用改变的体细胞核移植到灵长类动物的卵子中，希望产生多能干细胞，同时绕过在此过程中生产/破坏可行的胚胎。我们发现，灵长类胚胎中的滋养外胚层谱系可以通过下调未受精卵母细胞中的CDX 2来废除。多能性内细胞谱系不受影响，并产生功能性ES细胞。