Horizontal mtDNA Exchange
Horizontal mtDNA Exchange
批准号:
10356794
负责人:
SHOUKHRAT M MITALIPOV
金额:
$55.93万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-04-01 至 2024-01-31
关键词:
AddressAdultAgeAgingBiologyCell CommunicationCell LineageCell divisionCell physiologyCellsCellular biologyChimera organismCytoplasmic OrganelleDNADevelopmentDiabetes MellitusEpigenetic ProcessEtiologyFutureGeneticGenomeGoalsHaplotypesHistonesIndividualMalignant NeoplasmsMammalian CellMetabolicMitochondriaMitochondrial DNAMitoticMouse StrainsMusMutationNeurodegenerative DisordersNuclearOocytesOrganParentsPathogenicityPathologicPatientsPhysiological ProcessesProductionReactive Oxygen SpeciesResearchRoleSourceTestingTherapeuticTissuesage relatedagedbasedaughter cellembryo cellgenome integrityheteroplasmyhuman diseasein vivoinsightmitochondrial DNA mutationmitochondrial dysfunctionmitochondrial genomemutantoffspringrecombinational repairresponsesingle cell analysisstem cellstraffickingtransgenerational epigenetic inheritance
中文摘要
项目总结:
线粒体是细胞质细胞器,由于其在能量中的主要作用,对基本细胞功能至关重要
生产和线粒体功能障碍与许多人类疾病的病因学有关。它们包含
他们自己的基因组(MtDNA)是通过母体传播的。目前的教条认为哺乳动物细胞传递
在细胞分裂期间,它们的核和线粒体基因组完全垂直于子细胞。然而,
更出人意料的是,我们的初步研究表明,线粒体和
不是亲子关系的细胞之间的线粒体DNA。这项提议的目的是测试我们的
主要假设水平线粒体DNA转移在正常发育过程中很常见,对维持
或者拯救细胞的新陈代谢潜能。我们的团队试图揭开基因、表观遗传和细胞
管理水平线粒体DNA获取的机制,并找到关于如何、何时以及为什么在
捐献遗体并接受线粒体DNA。最终目标是了解线粒体dna在高能量需求下的稳定性。
器官和组织,尤其是在衰老过程中。我们的研究可能会对我们的
了解线粒体DNA生物学并指导未来治疗方法的发展以维持
通过实验性线粒体转移和替换来保持线粒体基因组的完整性。
英文摘要
Project Summary:
Mitochondria are cytoplasmic organelles, critical to basic cellular function due to their principal role in energy
production and mitochondrial dysfunction is implicated in the etiology of many human diseases. They contain
their own genome (mtDNA) that is transmitted maternally. Current dogma holds that mammalian cells transmit
their nuclear and mitochondrial genomes exclusively vertically to daughter cells, during cell divisions. However,
and rather unexpectedly, our preliminary studies have demonstrated horizontal exchange of mitochondria and
mtDNA between cells that are not in a parent-offspring relationship. The objective of this proposal is to test our
main hypothesis that horizontal mtDNA transfer is common during normal development and is critical to maintain
or rescue the metabolic potential of cells. Our team seeks to uncover genetic, epigenetic and cellular
mechanisms governing horizontal mtDNA acquisition and find answers on how, when and why cells within a
body donate and accept mtDNA. The ultimate goal is to understand mtDNA stability in high energy-demanding
organs and tissues, particularly during aging. Our research will likely have a significant impact on our
understanding of mtDNA biology and guide the development of future therapeutic approaches to maintain
mitochondrial genome integrity by experimental mitochondrial transfer and replacement.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Reconstructing Somatic Chromosomes
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批准号:10772559
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项目类别:
-
资助金额:$65.99万
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财政年份:2023
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Horizontal mtDNA Exchange
-
批准号:9902293
-
项目类别:
-
资助金额:$55.93万
-
财政年份:2019
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Horizontal mtDNA Exchange
-
批准号:10584513
-
项目类别:
-
资助金额:$55.93万
-
财政年份:2019
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Mitochondrial Aging and Reprogramming
-
批准号:9142455
-
项目类别:
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资助金额:$52.31万
-
财政年份:2015
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负责人:SHOUKHRAT M MITALIPOV
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依托单位:
CORRECTING MITOCHONDIRAL GENE MUTATIONS IN HUMAN OOCYTES
-
批准号:8357848
-
项目类别:
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资助金额:$5.82万
-
财政年份:2011
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
ALTERED NUCLEAR TRANSFER
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批准号:8357769
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项目类别:
-
资助金额:$14.55万
-
财政年份:2011
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
HISTOCOMPATIBLE PRIMATE EMBYONIC STEM CELLS (ESCS)
-
批准号:8357828
-
项目类别:
-
资助金额:$7.28万
-
财政年份:2011
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
DERIVING OOCYTES FROM EMBRYONIC STEM CELLS (ESCS)
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批准号:8357829
-
项目类别:
-
资助金额:$4.36万
-
财政年份:2011
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
MITOCHONDRIAL GENE THEREAPY IN A MACAQUE MODEL
-
批准号:8357849
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项目类别:
-
资助金额:$7.28万
-
财政年份:2011
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Altered Nuclear Transfer
-
批准号:8013894
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项目类别:
-
资助金额:$53.81万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
PRIMATE PLURIPOTENT CELLS FOR AUTOLOGOUS TRANSPLANTATION
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批准号:8173333
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项目类别:
-
资助金额:$7.61万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Mitochondrial Gene Therapy
-
批准号:8447582
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项目类别:
-
资助金额:$57.42万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Altered Nuclear Transfer
-
批准号:8431435
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项目类别:
-
资助金额:$52.91万
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财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
GENETIC ANALYSIS OF GERM CELL FORMATION
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批准号:8173332
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项目类别:
-
资助金额:$7.61万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Altered Nuclear Transfer
-
批准号:8212330
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项目类别:
-
资助金额:$54.36万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Mitochondrial Gene Therapy
-
批准号:8056813
-
项目类别:
-
资助金额:$61.86万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
DERIVING OOCYTES FROM ESCS
-
批准号:8173327
-
项目类别:
-
资助金额:$4.76万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Mitochondrial Gene Therapy
-
批准号:8241150
-
项目类别:
-
资助金额:$61.5万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Mitochondrial Gene Therapy
-
批准号:8644818
-
项目类别:
-
资助金额:$53.0万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
Mitochondrial Gene Therapy
-
批准号:7898222
-
项目类别:
-
资助金额:$64.35万
-
财政年份:2010
-
负责人:SHOUKHRAT M MITALIPOV
-
依托单位:
海外基金