NANO-CHIP HIV DETECTION IN VULNERABLE POPULATIONS

描述(由申请人提供)：我们建议开发一种新的干预模式，旨在协调和简化艾滋病毒治疗，这与老年人和其他流动性较差的人群特别相关。这一模式依赖于新型生物纳米传感器(BNS)诊断/治疗监测技术的开发，该技术将在家庭环境中实时为卫生工作者和患者提供有关疾病状况的重要医疗信息。这项新技术将有助于建立一个新的艾滋病毒/艾滋病管理系统，从而更有效地监测和治疗艾滋病毒感染及其并发症。这些目标是通过以下具体目标实现的：具体目标1：开发一种新型的用于检测HIV的压电式生物纳米传感器装置。在这个特定目标中，我们提出了一种基于生物标志物gp120和p24的存在以及完整的HIV病毒的快速(10-15分钟)液相检测HIV的压电式生物纳米传感器(BNS)装置。然后，我们将优化检测灵敏度、特异度和动态范围，并根据当前基于实验室的ELISA/SPR检测格式验证该设备。里程碑：发展了一种压电式BNS检测方法，能够在反应时间15分钟内检测到gp120和p24，检测极限为100pg/ml，同时使用不超过10-100&lt；L的流体样本。具体目标2：设计和开发与艾滋病毒/艾滋病相关的多个生物标志物的BNS检测一旦在特定目标1中确定了基本的BNS设备格式和艾滋病毒检测方法，我们将开发单独的生物芯片，用于检测艾滋病毒感染患者常规评估的其他生物标志物：包括CD4、胰岛素、C肽、IL-6和HbA1C，并将其应用于血液和血清分析。因此，这项BNS分析将提供艾滋病毒/艾滋病患者的具体健康概况。BNS优化将包括确定每个生物标志物的单独反应条件，以及相关的操作条件，如：电极大小、堆积密度、最佳尺寸、声学相互作用模式、用于监测多个传感器和信号处理的软件和硬件开发。里程碑：开发一种BNS系统，以皮克灵敏度同时检测生物标记物gp120、p24、CD4、胰岛素、C肽、IL-6和HbA1C，预计每个样本的检测成本为5-10美元 公共卫生相关性：目前针对感染艾滋病毒和其他疾病的老年患者(感染艾滋病毒的老年人数预计将在未来几年增加)的护理战略面临着与高度复杂的治疗模式相关的挑战，这些治疗模式缺乏协调，因此往往使护理效果降低。由于多种药物和多种问题，这些患者被迫忍受较低的生活质量，其特点是治疗并发症和医疗结果下降的可能性很高。为了满足这一需求，我们提出了一种干预模型(一种独创的生物纳米传感器检测技术)，旨在协调和简化艾滋病毒治疗，特别是在患有多种疾病的老年人中。

DESCRIPTION (provided by applicant): We propose to develop a new intervention model directed toward the coordination and simplification of HIV treatment which has particular relevance to older persons and other, less mobile, populations. This model relies on the development of a novel Bio-nanosensor (BNS) diagnostic/treatment monitoring technology that will provide both health workers as well as patients with important medical information regarding disease status on a real-time basis in the home setting. This new technology will enable the creation of a new HIV/AIDS management system that will result in more efficient and effective monitoring and treatment of HIV infection and its complications. These goals are achieved through the following specific aims: Specific Aim 1: Develop a Novel Piezoelectric Bio-nanosensor Device to Detect HIV In this specific aim, we propose to develop a piezoelectric Bio-nanosensor (BNS) device for the rapid (10-15 min) fluid-phase detection of HIV based on the presence of the biomarkers gp120 and p24, and of intact HIV virus. We will then optimize assay sensitivity, specificity and dynamic range, and validation this device against the current laboratory-based ELISA/SPR detection format. Milestone: The development of a piezoelectric BNS assay with the ability to detect gp120 and p24 in <15 min reaction time with a limit of detection <100 pg/ml, while using no more than 10 -100 <l of fluid sample. Specific Aim 2: Design and Develop a BNS assay for Multiple Biomarkers Associated with HIV/AIDS Once the design of the basic BNS device format and HIV detection methods are established in Specific Aim 1, we will develop individual biochips optimized for sensing additional biomarkers that are routinely assessed in HIV infected patients: including CD4, insulin, C-peptide, IL-6 and HbA1C, and apply these to blood and serum analysis. This BNS assay will thus provide a specific health profile of HIV/AIDS patients. BNS optimization will include determining individual reaction conditions for each biomarker, and relevant operating conditions such as: size of electrode, packing density, optimum dimensions, acoustic mode of interaction, software and hardware development for monitoring multiple sensors and signal processing. Milestone: Development of a BNS system for simultaneous detection of the biomarkers gp120, p24, CD4, insulin, C-peptide, IL-6 and HbA1C at picogram sensitivity with an anticipated assay cost of $5 - $10/sample PUBLIC HEALTH RELEVANCE: Current care strategies for older patients (the number of older persons with HIV infection is forecast to augment in the years to come) with HIV infection as well as other conditions face challenges associated with highly complex treatment paradigms that lack coordination and thus often render care less effective. With multiple drugs and multiple problems, such patients are forced to endure a lower quality of life marked by the high possibility of treatment complications and diminished medical outcomes. In order to address this need, we propose an intervention model (an original bio-nanosensor detection technology) directed toward the coordination and simplification of HIV treatment, particularly in older persons suffering from multiple conditions.