NANO-CHIP HIV DETECTION IN VULNERABLE POPULATIONS

描述（由申请人提供）：我们建议开发一种新的干预模式，旨在协调和简化艾滋病毒治疗，这与老年人和其他流动性较差的人群特别相关。这种模式依赖于一种新型生物纳米传感器（BNS）诊断/治疗监测技术的发展，该技术将为卫生工作者和患者在家庭环境中实时提供有关疾病状况的重要医疗信息。这项新技术将能够建立一种新的艾滋病毒/艾滋病管理系统，从而更有效地监测和治疗艾滋病毒感染及其并发症。具体目标1：开发一种新型压电生物纳米传感器设备来检测HIV。在这个具体目标中，我们提出开发一种压电生物纳米传感器（BNS）设备，用于基于生物标志物gp120和p24以及完整的HIV病毒的存在快速（10-15分钟）液相检测HIV。然后，我们将优化检测灵敏度、特异性和动态范围，并根据目前基于实验室的ELISA/SPR检测格式验证该设备。里程碑：开发了一种压电BNS检测方法，能够在<15分钟的反应时间内检测gp120和p24，检测限<100 pg/ml，同时使用不超过10 -100 <l的流体样品。特异性目标2：设计和开发与HIV/AIDS相关的多种生物标志物的BNS检测方法，一旦在特异性目标1中确定了基本BNS设备格式和HIV检测方法的设计，我们将开发针对检测HIV感染患者常规评估的其他生物标志物（包括CD4，胰岛素，c肽，IL-6和HbA1C）进行优化的单个生物芯片，并将其应用于血液和血清分析。因此，这种BNS检测将提供艾滋病毒/艾滋病患者的具体健康概况。BNS优化将包括确定每个生物标志物的单独反应条件，以及相关的操作条件，例如：电极尺寸、包装密度、最佳尺寸、相互作用的声学模式、用于监控多个传感器和信号处理的软件和硬件开发。里程碑：开发一种BNS系统，用于同时检测gp120、p24、CD4、胰岛素、c肽、IL-6和HbA1C生物标志物，具有picogram sensitivity，预计检测成本为5 - 10美元/个样品

DESCRIPTION (provided by applicant): We propose to develop a new intervention model directed toward the coordination and simplification of HIV treatment which has particular relevance to older persons and other, less mobile, populations. This model relies on the development of a novel Bio-nanosensor (BNS) diagnostic/treatment monitoring technology that will provide both health workers as well as patients with important medical information regarding disease status on a real-time basis in the home setting. This new technology will enable the creation of a new HIV/AIDS management system that will result in more efficient and effective monitoring and treatment of HIV infection and its complications. These goals are achieved through the following specific aims: Specific Aim 1: Develop a Novel Piezoelectric Bio-nanosensor Device to Detect HIV In this specific aim, we propose to develop a piezoelectric Bio-nanosensor (BNS) device for the rapid (10-15 min) fluid-phase detection of HIV based on the presence of the biomarkers gp120 and p24, and of intact HIV virus. We will then optimize assay sensitivity, specificity and dynamic range, and validation this device against the current laboratory-based ELISA/SPR detection format. Milestone: The development of a piezoelectric BNS assay with the ability to detect gp120 and p24 in <15 min reaction time with a limit of detection <100 pg/ml, while using no more than 10 -100 <l of fluid sample. Specific Aim 2: Design and Develop a BNS assay for Multiple Biomarkers Associated with HIV/AIDS Once the design of the basic BNS device format and HIV detection methods are established in Specific Aim 1, we will develop individual biochips optimized for sensing additional biomarkers that are routinely assessed in HIV infected patients: including CD4, insulin, C-peptide, IL-6 and HbA1C, and apply these to blood and serum analysis. This BNS assay will thus provide a specific health profile of HIV/AIDS patients. BNS optimization will include determining individual reaction conditions for each biomarker, and relevant operating conditions such as: size of electrode, packing density, optimum dimensions, acoustic mode of interaction, software and hardware development for monitoring multiple sensors and signal processing. Milestone: Development of a BNS system for simultaneous detection of the biomarkers gp120, p24, CD4, insulin, C-peptide, IL-6 and HbA1C at picogram sensitivity with an anticipated assay cost of $5 - $10/sample PUBLIC HEALTH RELEVANCE: Current care strategies for older patients (the number of older persons with HIV infection is forecast to augment in the years to come) with HIV infection as well as other conditions face challenges associated with highly complex treatment paradigms that lack coordination and thus often render care less effective. With multiple drugs and multiple problems, such patients are forced to endure a lower quality of life marked by the high possibility of treatment complications and diminished medical outcomes. In order to address this need, we propose an intervention model (an original bio-nanosensor detection technology) directed toward the coordination and simplification of HIV treatment, particularly in older persons suffering from multiple conditions.