Neurointensive Care and Assessment Facility

G20提案的总体目标是创建一个共享的神经重症监护和评估设施（NCAF），以研究大型动物疾病和损伤模型的急性和长期反应，从而开发和转化促进和保护神经功能的新治疗和技术。本修订提案具体和实质性地回应了初步审查中提出的每一项意见。NCAF将是一个独一无二的共享猪设施，用于研究猪/仔猪（体重25 kg）中风、心脏骤停、心肺转流和创伤性脑损伤后的神经损伤和恢复。该设施将由5个部分组成：（1）用于闭合性头部损伤和终末期手术的非无菌手术室，（2）重症监护室，（3）认知和行为评估实验室，（4）生存手术室和（5）动物准备区。将对设施进行升级，以允许对USDA监管的种属进行生存手术，并满足实验动物护理和使用指南（ILAR，1996;预出版，2010）的要求，并提供最先进的重症监护室，用于监测和圈养动物。额外的空间将被翻新，作为认知和行为评估的程序空间，使用由设施总监开发的一系列猪特异性测试。最后，NCAF设施将升级，以满足联邦和其他监管要求，确保安全，消毒，安全，害虫控制和环境控制的手术，实验程序，重症监护室和行为研究领域。NCAF将用于每年超过800头仔猪/猪的研究，并将由医学院（SOM）和工程与应用科学学院（SEAS）的25名研究人员共享，目前和待定的资金超过4200万美元。此外，这个新的设施将是一个独特的机会，以创造SOM教师之间的协同作用，研究细胞和分子的方法，以治疗干预措施，保留神经功能，与SEAS教师谁是创新者在设备开发，数据集成，数据处理，机器学习，生物传感器和微创手术技术。因此，这些革新将加强PHS资助的研究，将推进实验研究中的动物福利行为，并将对宾夕法尼亚大学临床前研究的范围和步伐产生积极影响。

DESCRIPTION (provided by applicant): The overall goal of this G20 proposal is to create a shared Neurointensive Care and Assessment Facility (NCAF) to study acute and long-term responses in large animal models of disease and injury to enable the development and translation of new treatments and technologies that promote and preserve neurological function. This revised proposal responds specifically and substantively to each comment raised in the initial review. The NCAF will be a one-of-a-kind shared porcine facility for the study of neurological injury and recovery after stroke, cardiac arrest, cardiopulmonary bypass, and traumatic brain injury in pigs/piglets d 25kg. The facility will consist of 5 components: (1) a non-sterile procedure room for closed head injuries and terminal procedures, (2) an intensive care housing room, (3) a cognitive and behavioral assessment laboratory, (4) a survival surgical room, and (5) animal prep area. Facilities will be upgraded to allow survival surgery on USDA-regulated species and meet requirements of the Guide for the Care and Use of Laboratory Animals (ILAR, 1996; pre-publication, 2010), and provide a state-of-the art intensive care unit for monitoring and housing animals. Additional space will be renovated to serve as procedural space for cognitive and behavioral assessment using a battery of porcine-specific tests developed by the Facility Director. Finally, the NCAF facility will be upgraded to meet federal and other regulatory requirements that ensure safety, sanitization, security, vermin control and environmental control for surgery, experimental procedure, intensive care housing and behavioral study areas. The NCAF will be used for the study of more than 800 piglets/pigs per year, and will be shared by 25 investigators spanning the School of Medicine (SOM) and School of Engineering and Applied Science (SEAS) with over $42, million in current and pending funding. Moreover, this new facility will be a unique opportunity to create synergy between SOM faculty investigating cell and molecular approaches to therapeutic interventions that preserve neurofunction, with SEAS faculty who are innovators in device development, data integration, data processing, machine learning, biosensors and minimally invasive surgical technology. Thus, these renovations will enhance PHS-funded research, will advance the conduct of animal welfare in experimental research, and will positively impact the scope and pace of the preclinical research at Penn.