COBRE: Membrane Protein Production and Characterization

超出空间 但前提是。 膜蛋白生产和特性的COMPPAC(COMPPAC)是由 膜蛋白在生物学中的关键作用与公认的在生物中的缺陷之间的差距 这些分子的生产和结构表征。我们将通过一套 五个分项目，涉及来自所有教员职级的四个学科的七个个人投资项目，所有五个 首席调查员要么是职业生涯早期的调查员，要么是初级调查员。这项研究的范围涵盖了来自 膜蛋白的过度表达、增溶和纯化，以结构功能研究为基础 结晶学特征，主要是生物医学上有意义或相关的功能研究 膜蛋白。要研究的膜蛋白跨越多种结构和功能。 特性，包括受体、黏附蛋白和界面酶，以及它们与 将检查几种重要的生物医学条件，包括癌症、精子发生和 化学战。科布雷将凭借内部的合作而具有强烈的跨学科色彩 在子项目之间，随着初始子项目获得独立的支持， 研究活动将通过在几个额外的活性膜内进行互补研究来扩大 蛋白质研究实验室。将为蛋白质生产、结构生物学和 生物成像与生物分子研究的实质性基础设施紧密结合在 特拉华大学和特拉华生物技术研究所。科布雷还将培育 正在开发的方法对膜蛋白的系统应用，特别是对膜蛋白的 表达、纯化及结构生物学研究。这可能会对 膜蛋白研究界的广泛关注，以及对膜蛋白国家基础设施的研究 NIH路线图中正在开发的结构生物学。

EXCEED THE SPACE PROVIDED. The COBRE on Membrane Protein Production and Characterization (COMPPAC) is motivated by the disparity between the critical role of membrane proteins in biology and the well-recognized deficiencies in production and structural characterization of these molecules. We will address this challenge via a suite of five subprojects involving seven Pis from four academic disciplines at all faculty ranks, with all five of the lead Pis being either early-career or junior investigators. The scope of the research spans a spectrumfrom membrane protein overexpression, solubilization and purification, to structure-function studies based on crystallographic characterization, to predominantly functional studies of biomedically significant or related membrane proteins. The membrane proteins to be studied span a variety of structural and functional characteristics, including receptors, adhesion proteins and interfacial enzymes, and their relevance to several biomedically important conditions will be examined, including cancer, spermatogenesis and chemical warfare. The COBRE will have a strong interdisciplinary flavor by virtue of collaborations within and between subprojects, and as the initial subprojects acquire independent support, the scope of the research activities will be expanded by complementary research within several additional active membrane protein research labs. Core facilities support to be provided for protein production, structural biology and bioimaging is closely integrated with the substantial infrastructure for biomolecular research at the University of Delaware and the Delaware Biotechnology Institute. The COBRE will also foster the systematic application of the methods under development to membrane proteins in general, especially for the expression, purification and structural biology studies. This can have a significant impact on the membrane protein research community at large, and on the national infrastructure on membrane protein structural biology being developed within the NIH Roadmap.