COBRE: Membrane Protein Production and Characterization

超出空间 提供了 COBRE膜蛋白生产和表征（COMPPAC）的动机是 膜蛋白在生物学中的关键作用和公认的缺陷之间的差距， 这些分子的生产和结构表征。我们将通过一系列措施来应对这一挑战， 五个次级计划，涉及来自四个学科的七名私人研究员， 首席私家侦探要么是职业初期要么是初级调查员研究的范围涵盖了从 膜蛋白过表达，溶解和纯化，结构功能研究的基础上， 晶体学表征，以生物医学意义或相关的主要功能研究 膜蛋白待研究的膜蛋白跨越多种结构和功能 特性，包括受体，粘附蛋白和界面酶，以及它们与 将检查几种生物医学上重要的情况，包括癌症、精子发生和 化学战COBRE将通过内部合作， 由于最初的子项目获得了独立的支助， 研究活动将通过几种额外活性膜的补充研究来扩大 蛋白质研究实验室为蛋白质生产、结构生物学和 生物成像与生物分子研究的实质性基础设施紧密结合， 特拉华州大学和特拉华州生物技术研究所。COBRE还将促进 将正在开发的方法系统地应用于一般的膜蛋白，特别是用于 表达、纯化和结构生物学研究。这可能会对 膜蛋白研究界在整个，并对国家基础设施的膜蛋白 结构生物学正在NIH路线图中开发。

EXCEED THE SPACE PROVIDED. The COBRE on Membrane Protein Production and Characterization (COMPPAC) is motivated by the disparity between the critical role of membrane proteins in biology and the well-recognized deficiencies in production and structural characterization of these molecules. We will address this challenge via a suite of five subprojects involving seven Pis from four academic disciplines at all faculty ranks, with all five of the lead Pis being either early-career or junior investigators. The scope of the research spans a spectrumfrom membrane protein overexpression, solubilization and purification, to structure-function studies based on crystallographic characterization, to predominantly functional studies of biomedically significant or related membrane proteins. The membrane proteins to be studied span a variety of structural and functional characteristics, including receptors, adhesion proteins and interfacial enzymes, and their relevance to several biomedically important conditions will be examined, including cancer, spermatogenesis and chemical warfare. The COBRE will have a strong interdisciplinary flavor by virtue of collaborations within and between subprojects, and as the initial subprojects acquire independent support, the scope of the research activities will be expanded by complementary research within several additional active membrane protein research labs. Core facilities support to be provided for protein production, structural biology and bioimaging is closely integrated with the substantial infrastructure for biomolecular research at the University of Delaware and the Delaware Biotechnology Institute. The COBRE will also foster the systematic application of the methods under development to membrane proteins in general, especially for the expression, purification and structural biology studies. This can have a significant impact on the membrane protein research community at large, and on the national infrastructure on membrane protein structural biology being developed within the NIH Roadmap.