FLUORESCENCE SPECTROPHOTOMETER IN NIR RANGE FOR BIOLOGICAL AND MEDICAL APPLICATIO

用于生物和医学应用的近红外范围荧光分光光度计

• 批准号: 8052140
• 负责人: Mikhail Y. Berezin
• 金额: $ 29.2万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至 2012-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052140
• 关键词: 10 year old; Area; Bacteria; Biological; Brain; Contrast Media; Detection; Development; Diagnostic; Dyes; Fluorescence; Fluorescent Probes; Funding; Gene Proteins; Gold; Healthcare; Image; Intervention; Light; Medical; Methods; Missouri; Molecular; Molecular Medicine; Optical Tomography; Optics; Penetration; Photons; Polymers; Proteins; Receptor Cell; Research; Research Personnel; Saints; Source; System; Therapeutic; Tissues; United States National Institutes of Health; Universities; Washington; cancer therapy; design; detector; flexibility; imaging modality; imaging probe; instrument; molecular imaging; nanoparticle; optical imaging; tool; tumor

DESCRIPTION (provided by applicant): Full realization of the benefits of recent advances in molecular medicine requires the development of molecular imaging probes that target specific cells, receptors, aberrant genes, and proteins for therapeutic and diagnostic interventions. As with other imaging modalities, optical imaging using near-infrared (NIR) light (700-1700 nm) promises to become an important tool in healthcare. The NIR region of the spectrum, also known as the optical windows, offers important advantages for photon penetration deep into biological tissue. For fundamental studies of NIR photons with biological tissue and the development of molecular fluorescent probes optically active in NIR, we request to upgrade our 10 year old fluorometer to a state-of-the-art fluorescence spectrophotometer specifically dedicated to NIR. The requested system will be equipped with NIR light sources and ultrasensitive detectors, fluorescence lifetime, and polarization modules, will be highly flexible and will satisfy the urgent need of 13 NIH funded researchers from three major universities in the Saint Louis area: Washington University in St. Louis, University of Missouri -St. Louis, and Saint Louis University. The research that will be supported by this instrument includes diverse fields from the design of optically diverse contrast agents (organic dyes, polymer nanoparticles, gold nanocages, and fluorescent proteins), development of imaging modalities (fluorescence steady-state and lifetime imaging, photoacoustics, and optical tomography), to application of the probes and methods to tumor diagnostics, cancer treatment, bacteria detection, and understanding of brain function.

描述（由申请人提供）：要充分实现分子医学最新进展的益处，需要开发靶向特定细胞、受体、异常基因和蛋白质的分子成像探针，用于治疗和诊断干预。与其他成像方式一样，使用近红外（NIR）光（700-1700 nm）的光学成像有望成为医疗保健中的重要工具。光谱的NIR区域，也称为光学窗口，为光子深入生物组织提供了重要的优势。为了对生物组织的近红外光子进行基础研究以及开发在近红外中具有光学活性的分子荧光探针，我们要求将已有10年历史的荧光计升级为专门用于近红外的最先进的荧光分光光度计。所要求的系统将配备近红外光源和超灵敏检测器、荧光寿命和偏振模块，将具有高度灵活性，并将满足来自圣刘易斯地区三所主要大学的13名NIH资助研究人员的迫切需求：圣路易斯华盛顿大学、密苏里州大学圣路易斯分校和圣刘易斯大学。该仪器将支持的研究包括从光学多样化造影剂的设计到不同领域（有机染料、聚合物纳米颗粒、金纳米笼和荧光蛋白）、成像方式的开发（荧光稳态和寿命成像、光声和光学层析成像），涉及探针和方法在肿瘤诊断、癌症治疗、细菌检测以及对大脑功能的理解。

项目成果

Visualization of pulmonary clearance mechanisms via noninvasive optical imaging validated by near-infrared flow cytometry.

通过近红外流式细胞术验证的无创光学成像可视化肺部清除机制。

• DOI: 10.1002/cyto.a.22658
• 发表时间: 2015
• 期刊: Cytometry. Part A : the journal of the International Society for Analytical Cytology
• 作者: Zhou,Haiying;Gunsten,SeanP;Zhegalova,NataliaG;Bloch,Sharon;Achilefu,Samuel;ChristopherHolley,J;Schweppe,Daniel;Akers,Walter;Brody,StevenL;Eades,WilliamC;Berezin,MikhailY
• 通讯作者: Berezin,MikhailY

Highly sensitive image-derived indices of water-stressed plants using hyperspectral imaging in SWIR and histogram analysis.

• DOI: 10.1038/srep15919
• 发表时间: 2015-11-04
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Kim DM;Zhang H;Zhou H;Du T;Wu Q;Mockler TC;Berezin MY
• 通讯作者: Berezin MY