FACSAria II Cell Sorter

描述（由申请人提供）：为了满足多色流式细胞术细胞分选的巨大需求，LIAI正在申请资金，以将制造商不再支持的Becton Dickinson（BD; BD Biosciences圣何塞，CA）FACS Vantage SE更换为9色和2散射参数数字BD FACSAria II细胞分选仪。由于一个致力于免疫系统研究的研究所的令人难以置信的需求，该设施已经以接近最大容量运行，只有用更快，更有能力的设备替换该仪器，该设施才能够完全支持研究计划。选择所要求的仪器是因为它可以同时分析多达11个参数，并将显著增强同时评估细胞的许多表型参数的能力，以及大大提高当前仪器的样品通量/效率。重要的是，这款高速仪器将最大限度地提高从高细胞计数样本中分选的机会，并捕获通常在技术上具有挑战性的稀有细胞群。随着一大群极高容量的用户，更换现有仪器还将充分利用将常规样品与生物危害样品完全隔离的能力，生物危害样品将在专用房间和通风橱中的现有单元上运行组合.这种隔离将大大提高安全和调度效率，预计每年将使设施分拣能力增加近1,000小时;迫切需要100多名NIH支持的调查人员的支持。

DESCRIPTION (provided by applicant): In order to address the overwhelming demand for multi-color flow cytometry cell sorting, LIAI is requesting funds for the replacement of a Becton Dickinson (BD; BD Biosciences San Jose, CA) FACS Vantage SE that the manufacturer will no longer support, with a 9-color and 2 scatter parameter digital BD FACSAria II cell sorter. The facility already operates at near maximum capacity due to incredible demand from an institute devoted to the study of the immune system, and only by replacement of this instrument with a faster and more capable unit will the facility be able to fully support the research program. The requested instrument has been chosen because it can analyze up to 11 parameters simultaneously and will significantly enhance the ability to evaluate numerous phenotypic parameters of a cell concurrently, as well as greatly increased sample throughput/efficiency over the current instrument. Importantly, this high- speed instrument will maximize the opportunity to sort from high cell count samples and capture rare cell populations that are often technically challenging to collect. With a large group of extraordinarily high volume users, replacement of the current instrument will also leverage the ability to fully segregate conventional samples from biohazardous samples that will be run on an existing unit in a purpose built room and hood combination. This segregation will greatly increase safety and scheduling efficiencies that in total are expected to increase the facilities sorting capacity by nearly 1,000 hours per year; an urgent need in the support of over 100 NIH-supported investigators.