CORE--Animal

该项目的目的是了解高脂肪饮食是如何导致 捍卫体重。我们的总体战略是在多个层面上探索饮食性肥胖的模型， 从多个角度。动物和生理学核心整合并集中生产 肥胖大鼠及其各个对照组进行每个具体项目的研究。具体来说，这个核心 生成每个特定项目中使用的每种动物，从而确保每个 实验喜欢以相同方式对待的动物，从而允许比较 跨越时间、群体和项目。该核心有四个主要目标。1)生成必要的 动物的各个项目中的任何一个详细描述的各种饮食组中， 提案的导言部分。这些小组包括大鼠的低脂肪，半纯化黄油为基础的 饮食（LF），接受匹配的高脂肪、半纯化饮食（HF）的大鼠，接受HF饮食但具有卡路里的大鼠 与LF大鼠（PHF）的摄入量匹配（成对喂养），接受LF饮食和 与PHF相同的量：成对喂养大鼠（PLF）和颗粒饲料大鼠（CHOW）。核还 产生基于橄榄油而不是黄油的HF和LF饮食的对照大鼠。2)发展 每个项目所需的新型或改良的大鼠手术技术。3)开展 与适口性和膳食模式分析相关的行为方面的实验以及 能量消耗测量，以确定不同的脂肪酸如何影响消耗， 与体重调节相关的行为。4)进行胴体脂肪含量分析 每个项目的主题。这包括非侵入性（即，NMR读数）和 对整个胴体或特定解剖学子区域的最终评估（例如，皮下脂肪）。 最后，动物和生理学核心将进行某些试点或其他必要的实验， 创建与其他项目相关且至关重要的数据。动物/生理学核心人员 已经熟练掌握了迄今为止用于描述模型特征的所有技术; 因此，工作人员已经受过良好的培训，协议已经到位， 实现这一核心目标。

The program project is aimed at understanding how diets high in fat lead to increased levels of defended body weight. Our overall strategy is to explore a model of dietary obesity at many levels and from multiple perspectives. The Animal & Physiology Core consolidates and centralizes the production of obese rats and their various control groups for each of the specific projects. Specifically, this core generates EVERY animal that is used in each of the specific projects and thereby assures that each experiment enjoys animals that have been treated in an identical manner, thus allowing comparisons across time, cohorts and projects. There are four primary goals of this core. 1) To generate requisite animals for the individual projects in any of the various dietary groups described in detail in the introductory section to the proposal. These groups include rats on a low-fat, semi-purified butter oil-based diet (LF), rats on a matched high-fat, semi-purified diet (HF), rats receiving the HF diet but with calories matched (pair-fed) to intake of the LF rats (PHF), rats receiving the LF diet and food-restricted by the same amount as the PHF: pair fed rats (PLF), and rats with pelleted chow (CHOW). The Core also generates control rats on HF and LF diets based on olive oil as opposed to butter oil. 2) To develop novel or modified rat surgical techniques as required by each of the individual projects. 3) To carry out experiments on behavioral aspects associated with the palatability and meal pattern analysis as well energy expenditure measurements to determine how the different fatty acids influence consumption and behaviors associated with body weight regulation. 4) To carry out carcass analysis for body lipid content on subjects from each of the individual projects. This includes non-invasive (i.e., NMR readings) and terminal assessments for the entire carcass or specific anatomical subareas (e.g., subcutaneous fat). Lastly, The Animal & Physiology Core will carry out certain pilot or other experiments necessary for creating data that are relevant and critical to the other projects. The Animal/Physiology Core personnel are already facile with all of the techniques required to date that are used to characterize the model; consequently the personnel are already well-trained and the protocols are all in place to efficiently accomplish the goals of this essential core.