CORE--Animal

该计划项目旨在了解高脂肪饮食是如何导致体内 捍卫体重。我们的总体战略是探索多层次和多层次的饮食肥胖模式 从多个角度来看。动物和生理学核心巩固和集中生产 肥胖大鼠及其各个特定项目的不同对照组。具体地说，这个核心 生成在每个特定项目中使用的每种动物，从而确保每个 实验喜欢以同样的方式对待动物，从而允许进行比较 跨越时间、人群和项目。这个核心有四个主要目标。1)生成必需的 中详细描述的任何不同饮食组中的单个项目的动物 提案的导言部分。这些组包括以低脂肪、半纯化黄油为基础的大鼠 饮食(LF)，给予匹配的高脂肪半纯饮食(HF)的大鼠，接受HF饮食但有卡路里的大鼠 与LF大鼠(PHF)的摄入量相匹配(配对喂养)，接受LF饮食和食物限制的大鼠 剂量与PHF相同：配对喂养的大鼠(PLF)和颗粒饲料喂养的大鼠(Chow)。核心也是 以橄榄油为基础的HF和LF饮食产生对照大鼠，而不是黄油。2)发展 根据每个单独项目的需要，采用新的或改进的大鼠手术技术。3)开展 与适口性和进餐模式分析相关的行为方面的实验 能量消耗测量，以确定不同的脂肪酸如何影响消费和 与体重调节相关的行为。4)对体脂含量进行屠体分析 关于每个单独项目的主题。这包括非侵入性(即核磁共振读数)和 对整个屠体或特定解剖亚区(例如皮下脂肪)的最终评估。 最后，动物与生理学核心将进行某些试验或其他必要的实验 创建与其他项目相关且至关重要的数据。动物/生理学核心人员 已经熟练掌握了到目前为止用来描述模型特征的所有技术； 因此，工作人员已经接受了良好的培训，协议都已到位，以有效地 完成这一重要核心的目标。

The program project is aimed at understanding how diets high in fat lead to increased levels of defended body weight. Our overall strategy is to explore a model of dietary obesity at many levels and from multiple perspectives. The Animal & Physiology Core consolidates and centralizes the production of obese rats and their various control groups for each of the specific projects. Specifically, this core generates EVERY animal that is used in each of the specific projects and thereby assures that each experiment enjoys animals that have been treated in an identical manner, thus allowing comparisons across time, cohorts and projects. There are four primary goals of this core. 1) To generate requisite animals for the individual projects in any of the various dietary groups described in detail in the introductory section to the proposal. These groups include rats on a low-fat, semi-purified butter oil-based diet (LF), rats on a matched high-fat, semi-purified diet (HF), rats receiving the HF diet but with calories matched (pair-fed) to intake of the LF rats (PHF), rats receiving the LF diet and food-restricted by the same amount as the PHF: pair fed rats (PLF), and rats with pelleted chow (CHOW). The Core also generates control rats on HF and LF diets based on olive oil as opposed to butter oil. 2) To develop novel or modified rat surgical techniques as required by each of the individual projects. 3) To carry out experiments on behavioral aspects associated with the palatability and meal pattern analysis as well energy expenditure measurements to determine how the different fatty acids influence consumption and behaviors associated with body weight regulation. 4) To carry out carcass analysis for body lipid content on subjects from each of the individual projects. This includes non-invasive (i.e., NMR readings) and terminal assessments for the entire carcass or specific anatomical subareas (e.g., subcutaneous fat). Lastly, The Animal & Physiology Core will carry out certain pilot or other experiments necessary for creating data that are relevant and critical to the other projects. The Animal/Physiology Core personnel are already facile with all of the techniques required to date that are used to characterize the model; consequently the personnel are already well-trained and the protocols are all in place to efficiently accomplish the goals of this essential core.