BIOMARKERS ANALYSIS OF PREGNANT RHESUS MONKEYS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这项建议的目的是获得怀孕恒河猴的控制数据，并收集血液和粪便样本进行分析。这项研究将为先前在耶克斯进行的一项研究提供一个阴性对照组，该研究涉及在妊娠晚期挑战携带单核细胞增多性乳杆菌的怀孕恒河猴。在这项建议中，怀孕的猴子将被给予消毒的生奶油(车辆)，并在不同的时间间隔采集血液和粪便样本，以确定是否没有李斯特菌感染，同时收集血液单个核淋巴细胞的免疫反应参数。在最后一次采集血液样本后，母鸡和后代都被送回了殖民地。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this proposal is to obtain control data from pregnant rhesus monkeys and to collect blood and fecal samples for analysis. This study will provide a negative control group for a previous study performed at Yerkes that involved challenge of pregnant rhesus macaques with L. monocytogenes during late gestation. In this proposal pregnant monkeys will be given sterilized whipped cream (vehicle) and collected blood and fecal samples at various intervals to ascertain lack of Listeria infection while collecting immune response parameters from blood mononuclear lymphocytes. Both the dam and offspring are returned to the colony after the last blood sample collection.