Creating a Genetically Encodable Nano-Compass to Navigate in Molecular Networks
创建可遗传编码的纳米罗盘以在分子网络中导航
基本信息
- 批准号:8133978
- 负责人:
- 金额:$ 81.18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-09-30 至 2012-07-31
- 项目状态:已结题
- 来源:
- 关键词:BehaviorBiochemicalBiochemical ProcessBiologicalBiophysical ProcessCellsChemicalsDiseaseEngineeringEventGenerationsGoalsImageIn SituLeadLengthLifeMagnetismMaintenanceMeasurementMeasuresMechanicsMethodologyMolecularProcessPropertySignal PathwaySignal TransductionSystemTechniquesTechnologyTimeWorkabstractingcell behaviordesigninsightnanonovelpublic health relevanceresponsesingle moleculetool
项目摘要
DESCRIPTION
Abstract
Understanding how various biomolecules embedded within biochemical and mechanical networks are coordinated over multiple length and time scales to produce orchestrated cell behaviors requires characterization of their biochemical and biophysical properties in their native cellular context. However this represents a major challenge and our current understanding of the native behaviors of biomolecules has been limited by the lack of suitable techniques to measure and manipulate their dynamic properties in living cells. For example, to reveal causal connections and achieve a systems understanding of the signal transduction networks in living cells, a large set of molecular tools, designed to specifically perturb signaling pathways in situ and to quantitatively measure the cellular response, must be developed. Furthermore, very little is known about the mechanical properties and behaviors of molecules involved in force generation, cellular mechanics maintenance and mechanotransduction in a living cell due, in large part, to the lack of experimental methodologies for intracellular measurement and manipulation of biophysical properties. I propose to develop such molecular tools as genetically encodable magnetic probes by integrating molecular engineering strategies, chemical and cell biological approaches, and state-of-the-art biophysical measurement and manipulation combining single-molecule imaging and magnetic tweezers. This work will identify new strategies for simultaneous measurement and manipulation of intracellular molecular events and will provide enabling technologies for probing biochemical and biophysical processes in living cells. A better understanding of life processes at the molecular level will lead to new insights in treating diseases that result from dysregulation of these processes.
Public Health Relevance
The goal of this application is to develop novel magnetic probes that can be genetically encoded to enable new ways of measuring and manipulat
描述
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.
- DOI:10.1146/annurev-biochem-060409-093259
- 发表时间:2011
- 期刊:
- 影响因子:16.6
- 作者:Mehta S;Zhang J
- 通讯作者:Zhang J
Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.
- DOI:10.1021/cr100002u
- 发表时间:2011-05-11
- 期刊:
- 影响因子:62.1
- 作者:Newman, Robert H.;Fosbrink, Matthew D.;Zhang, Jin
- 通讯作者:Zhang, Jin
Luminescent kinase activity biosensors based on a versatile bimolecular switch.
- DOI:10.1021/ja1117396
- 发表时间:2011-04-20
- 期刊:
- 影响因子:15
- 作者:Herbst, Katie J.;Allen, Michael D.;Zhang, Jin
- 通讯作者:Zhang, Jin
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jin Zhang其他文献
Jin Zhang的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jin Zhang', 18)}}的其他基金
Integrating multi-omics, imaging, and longitudinal data to predict radiation response in cervical cancer
整合多组学、成像和纵向数据来预测宫颈癌的放射反应
- 批准号:
10734702 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
HPV genomic structure in cervical cancer radiation response and recurrence detection
HPV基因组结构在宫颈癌放射反应和复发检测中的作用
- 批准号:
10634999 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Deep learning in cervical cancer radiogenomics
宫颈癌放射基因组学中的深度学习
- 批准号:
10643978 - 财政年份:2022
- 资助金额:
$ 81.18万 - 项目类别:
Deep learning in cervical cancer radiogenomics
宫颈癌放射基因组学中的深度学习
- 批准号:
10424854 - 财政年份:2022
- 资助金额:
$ 81.18万 - 项目类别:
HPV alternative splicing in cervical cancer radiation response
HPV选择性剪接在宫颈癌放射反应中的作用
- 批准号:
10308435 - 财政年份:2020
- 资助金额:
$ 81.18万 - 项目类别:
HPV alternative splicing in cervical cancer radiation response
HPV选择性剪接在宫颈癌放射反应中的作用
- 批准号:
9891761 - 财政年份:2020
- 资助金额:
$ 81.18万 - 项目类别:
HPV alternative splicing in cervical cancer radiation response
HPV选择性剪接在宫颈癌放射反应中的作用
- 批准号:
10523104 - 财政年份:2020
- 资助金额:
$ 81.18万 - 项目类别:
FASEB SRC on Protein Kinases and Protein Phosphorylation
FASEB SRC 关于蛋白激酶和蛋白磷酸化
- 批准号:
9754337 - 财政年份:2019
- 资助金额:
$ 81.18万 - 项目类别:
相似海外基金
CAREER: Biochemical and Structural Mechanisms Controlling tRNA-Modifying Metalloenzymes
职业:控制 tRNA 修饰金属酶的生化和结构机制
- 批准号:
2339759 - 财政年份:2024
- 资助金额:
$ 81.18万 - 项目类别:
Continuing Grant
Leveraging releasable aryl diazonium ions to probe biochemical systems
利用可释放的芳基重氮离子探测生化系统
- 批准号:
2320160 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Standard Grant
Diurnal environmental adaptation via circadian transcriptional control based on a biochemical oscillator
基于生化振荡器的昼夜节律转录控制的昼夜环境适应
- 批准号:
23H02481 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Systematic manipulation of tau protein aggregation: bridging biochemical and pathological properties
tau 蛋白聚集的系统操作:桥接生化和病理特性
- 批准号:
479334 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Operating Grants
Converting cytoskeletal forces into biochemical signals
将细胞骨架力转化为生化信号
- 批准号:
10655891 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Enhanced Biochemical Monitoring for Aortic Aneurysm Disease
加强主动脉瘤疾病的生化监测
- 批准号:
10716621 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Biochemical Mechanisms for Sustained Humoral Immunity
持续体液免疫的生化机制
- 批准号:
10637251 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Structural and biochemical investigations into the mechanism and evolution of soluble guanylate cyclase regulation
可溶性鸟苷酸环化酶调节机制和进化的结构和生化研究
- 批准号:
10604822 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Chemical strategies to investigate biochemical crosstalk in human chromatin
研究人类染色质生化串扰的化学策略
- 批准号:
10621634 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Examination of risk assessment and biochemical assessment of fracture development focusing on the body composition of patients with rheumatoid arthritis
关注类风湿性关节炎患者身体成分的骨折发生风险评估和生化评估检查
- 批准号:
22KJ2600 - 财政年份:2023
- 资助金额:
$ 81.18万 - 项目类别:
Grant-in-Aid for JSPS Fellows