PROTEOMICS AND GENOMICS CORE FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 注：表格和数字与计划摘要一起上载。 A.具体目标 基因组与蛋白质组学和BL3-Flow Cytometer(FACS)Core是基因组和蛋白质组学核心与Flow Cytotics/BL3 Core管理性结合的产物。联合基因组和蛋白质组学及BL3-FACS核心的使命是为研究人员、研究生和研究支持人员提供技术支持和服务，以进行基因和蛋白质的创新研究，特别是支持妇女健康、艾滋病毒-艾滋病和相关领域的研究。 基因组学和蛋白质组学和BL3-FACS)核心是多功能的，因为该核心提供了对基因和蛋白质表达进行实验和/或使用流式细胞术的设备和技术专长、用于数据分析和数据共享(即生物信息学)的计算资源以及访问现有文献和数据库以促进实验设计。Meharry中心的具体职能是：1)为Meharry医学院的研究人员、研究生和研究支持人员提供必要的专家人员和设备，以提供自动DNA测序、DNA微阵列分析、实时聚合酶链式反应、BL3访问和流式细胞术等服务；2)储存用于研究的试剂库存，并促进分子生物试剂的购买；3)在使用设施内的共用设备方面提供技术援助和培训；4)为基因组和蛋白质组数据的分析提供技术专长、硬件和软件；以及5)为教职员工、研究生和博士后研究员提供关于基因组学和蛋白质组学(以及FACS等仪器的使用)等主题的培训计划。CORE的最终目标是提供梅哈里研究社区所需的服务，以提高研究生产率，这体现在教师拨款申请和获得的增加，以及教师发表论文数量的增加。 总之，基因组学和蛋白质组学以及BL3-FACS Core的主要目的是持续提供服务，以增强Meharry医学院研究人员进行重大生物医学研究的能力。基因组学和蛋白质组学和BL3-FACS)核心的主要服务活动是快速和经济高效地提供准确的DNA序列信息，获得FACS分析和BL3级生物遏制，通过使用核心中的共享设备提供技术援助，并提供方便的分子生物试剂。该组件的具体目标是： 1.为梅哈里医学院的教职员工和学生提供必要的专家人员和设备，以提供自动DNA测序、DNA微阵列分析、实时聚合酶链式反应、BL3接入和流式细胞术等服务。 2.在使用基因组和蛋白质组学和BL3-FACS核心的共用设备方面提供技术援助。 3.为基因组和蛋白质组数据的实验设计和分析提供技术专长、硬件和软件。 4.为教职员工和学生提供基因组学和蛋白质组学方面的培训以及流式细胞术的应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NOTE: TABLES AND FIGURES WERE UPLOADED WITH PROGRAM SUMMARY. A. Specific Aims The Genomics and Proteomics and BL3-Flow Cytometry (FACS) Core resulted from administratively combining the Genomics and Proteomics Core and the Flow Cytometry/BL3 Core. The mission of the combined Genomics & Proteomics & BL3-FACS Core is to provide the technical support and services for research faculty, graduate students and research support staff to perform innovative research on genes and proteins with special emphasis on the support of research in the areas of women health, HIV-AIDS, and related fields. The Genomics and Proteomics and BL3-FACS) Core is multi-functional since this Core provides the equipment and technical expertise to perform experiments on gene and protein expression and/or using flow cytometry, the computational resources for data analysis and data sharing (i.e., bioinformatics) and access to existing literature and databases to facilitate experimental design. The specific functions of this Core at Meharry are: 1) to provide to the research faculty, graduate students and research support staff of Meharry Medical College the necessary expert personnel and equipment to provide the services of automated DNA sequencing, DNA microarray analysis, real-time PCR, BL3 access and flow cytometry; 2) to stock an inventory of reagents for research and facilitate the purchase of molecular biological reagents; 3) to provide technical assistance and training in the use of shared equipment housed in the facility; 4) to provide the technical expertise, hardware and software for the analysis of genomic and proteomic data; and 5) to provide a program of training on topics such as genomics and proteomics (and use of instruments such as FACS) for faculty, staff, graduate students and post-doctoral fellows. The ultimate goal of the Core is to provide services needed by the research community at Meharry to increase research productivity as evidenced by increased faculty grant submissions and acquisitions, and increased publication numbers by faculty. In summary, the primary purpose of the Genomics and Proteomics and BL3-FACS Core is to provide on a continuing basis services to enhance the capability of the researchers at Meharry Medical College to perform significant biomedical research. The major service activities of the Genomics and Proteomics and BL3-FACS) Core are to provide accurate DNA sequence information rapidly and cost-effectively, access to FACS analysis and BL3 level biological containment, provide technical assistance with the use of shared equipment housed in the Core, and provide convenient access to molecular biological reagents. The specific goals for this component are: 1. To provide to the faculty, students and staff of Meharry Medical College the necessary expert personnel and equipment to provide the services of automated DNA sequencing, DNA microarray analysis, real-time PCR, BL3 access and flow cytometry. 2. To provide technical assistance with the use of shared equipment housed in the Genomics and Proteomics and BL3-FACS Core. 3. To provide the technical expertise, hardware and software for the experimental design and analysis of genomic and proteomic data. 4. To provide a program of training in genomics and proteomics and the utility of flow cytometry for faculty, staff and students.