PROTEOMICS AND GENOMICS CORE FACILITY

蛋白质组学和基因组学核心设施

• 批准号: 8357133
• 负责人: ROBERT G HOLT
• 金额: $ 29.2万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357133
• 关键词: Acquired Immunodeficiency Syndrome; Area; Bioinformatics; Biological; Biological Containment; Biomedical Research; Communities; Computer software; Core Facility; DNA Microarray Chip; DNA Sequence; Data; Data Analyses; Databases; Equipment; Equipment and supply inventories; Experimental Designs; Faculty; Flow Cytometry; Funding; Gene Expression; Gene Proteins; Genomics; Goals; Grant; HIV; Health Services Research; Housing; Human Resources; Literature; Microarray Analysis; Mission; Molecular; National Center for Research Resources; Postdoctoral Fellow; Principal Investigator; Productivity; Proteomics; Publications; Reagent; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Services; Source; Students; Technical Expertise; Time; Training; Training Programs; United States National Institutes of Health; Women' s Health; base; computing resources; cost; data sharing; experimental analysis; graduate student; innovation; instrument; medical schools; programs; protein expression; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NOTE: TABLES AND FIGURES WERE UPLOADED WITH PROGRAM SUMMARY. A. Specific Aims The Genomics and Proteomics and BL3-Flow Cytometry (FACS) Core resulted from administratively combining the Genomics and Proteomics Core and the Flow Cytometry/BL3 Core. The mission of the combined Genomics & Proteomics & BL3-FACS Core is to provide the technical support and services for research faculty, graduate students and research support staff to perform innovative research on genes and proteins with special emphasis on the support of research in the areas of women health, HIV-AIDS, and related fields. The Genomics and Proteomics and BL3-FACS) Core is multi-functional since this Core provides the equipment and technical expertise to perform experiments on gene and protein expression and/or using flow cytometry, the computational resources for data analysis and data sharing (i.e., bioinformatics) and access to existing literature and databases to facilitate experimental design. The specific functions of this Core at Meharry are: 1) to provide to the research faculty, graduate students and research support staff of Meharry Medical College the necessary expert personnel and equipment to provide the services of automated DNA sequencing, DNA microarray analysis, real-time PCR, BL3 access and flow cytometry; 2) to stock an inventory of reagents for research and facilitate the purchase of molecular biological reagents; 3) to provide technical assistance and training in the use of shared equipment housed in the facility; 4) to provide the technical expertise, hardware and software for the analysis of genomic and proteomic data; and 5) to provide a program of training on topics such as genomics and proteomics (and use of instruments such as FACS) for faculty, staff, graduate students and post-doctoral fellows. The ultimate goal of the Core is to provide services needed by the research community at Meharry to increase research productivity as evidenced by increased faculty grant submissions and acquisitions, and increased publication numbers by faculty. In summary, the primary purpose of the Genomics and Proteomics and BL3-FACS Core is to provide on a continuing basis services to enhance the capability of the researchers at Meharry Medical College to perform significant biomedical research. The major service activities of the Genomics and Proteomics and BL3-FACS) Core are to provide accurate DNA sequence information rapidly and cost-effectively, access to FACS analysis and BL3 level biological containment, provide technical assistance with the use of shared equipment housed in the Core, and provide convenient access to molecular biological reagents. The specific goals for this component are: 1. To provide to the faculty, students and staff of Meharry Medical College the necessary expert personnel and equipment to provide the services of automated DNA sequencing, DNA microarray analysis, real-time PCR, BL3 access and flow cytometry. 2. To provide technical assistance with the use of shared equipment housed in the Genomics and Proteomics and BL3-FACS Core. 3. To provide the technical expertise, hardware and software for the experimental design and analysis of genomic and proteomic data. 4. To provide a program of training in genomics and proteomics and the utility of flow cytometry for faculty, staff and students.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 注：表和图与试验总结一起上传。 A. 具体目标 基因组学和蛋白质组学和BL 3-流式细胞术（FACS）核心是通过基因组学和蛋白质组学核心与流式细胞术/BL 3核心的行政组合产生的。联合基因组学和蛋白质组学和BL 3-FACS核心的使命是为研究人员，研究生和研究支持人员提供技术支持和服务，以进行基因和蛋白质的创新研究，特别强调支持妇女健康，艾滋病毒/艾滋病和相关领域的研究。 基因组学和蛋白质组学和BL 3-FACS）核心是多功能的，因为该核心提供了设备和技术专业知识来执行关于基因和蛋白质表达和/或使用流式细胞术的实验、用于数据分析和数据共享的计算资源（即，生物信息学）和访问现有文献和数据库以促进实验设计。该中心的具体职能是：1）为Meharry医学院的研究人员，研究生和研究支持人员提供必要的专家人员和设备，以提供自动DNA测序，DNA微阵列分析，实时PCR，BL 3访问和流式细胞术的服务;（2）储存研究用试剂，并为购买分子生物试剂提供便利;（3）提供技术援助和培训，指导如何使用设施内的共用设备; 4）提供技术专长，硬件和软件的基因组和蛋白质组数据的分析;和5）提供一个培训计划的主题，如基因组学和蛋白质组学（和使用仪器，如流式细胞仪）的教师，工作人员，研究生和博士后研究员。核心的最终目标是提供所需的研究社区在梅哈里提高研究生产力的服务，通过增加教师补助金提交和收购证明，并通过教师增加出版物数量。 总之，基因组学和蛋白质组学和BL 3-FACS核心的主要目的是提供持续的服务，以提高Meharry医学院研究人员进行重要生物医学研究的能力。基因组学和蛋白质组学以及BL 3-FACS核心的主要服务活动是快速和经济有效地提供准确的DNA序列信息，获得FACS分析和BL 3级生物防护，提供使用核心中共享设备的技术援助，并提供方便的分子生物试剂。这一部分的具体目标是： 1.为Meharry医学院的教师，学生和工作人员提供必要的专家人员和设备，以提供自动DNA测序，DNA微阵列分析，实时PCR，BL 3访问和流式细胞术的服务。 2.为使用基因组学和蛋白质组学以及BL 3-FACS核心中的共享设备提供技术援助。 3.为基因组和蛋白质组数据的实验设计和分析提供技术专长、硬件和软件。 4.为教职员工和学生提供基因组学和蛋白质组学以及流式细胞术实用性的培训计划。