PROTEOMICS AND GENOMICS CORE FACILITY

蛋白质组学和基因组学核心设施

• 批准号: 8357133
• 负责人: ROBERT G HOLT
• 金额: $ 29.2万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357133
• 关键词: Acquired Immunodeficiency Syndrome; Area; Bioinformatics; Biological; Biological Containment; Biomedical Research; Communities; Computer software; Core Facility; DNA Microarray Chip; DNA Sequence; Data; Data Analyses; Databases; Equipment; Equipment and supply inventories; Experimental Designs; Faculty; Flow Cytometry; Funding; Gene Expression; Gene Proteins; Genomics; Goals; Grant; HIV; Health Services Research; Housing; Human Resources; Literature; Microarray Analysis; Mission; Molecular; National Center for Research Resources; Postdoctoral Fellow; Principal Investigator; Productivity; Proteomics; Publications; Reagent; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Services; Source; Students; Technical Expertise; Time; Training; Training Programs; United States National Institutes of Health; Women' s Health; base; computing resources; cost; data sharing; experimental analysis; graduate student; innovation; instrument; medical schools; programs; protein expression; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NOTE: TABLES AND FIGURES WERE UPLOADED WITH PROGRAM SUMMARY. A. Specific Aims The Genomics and Proteomics and BL3-Flow Cytometry (FACS) Core resulted from administratively combining the Genomics and Proteomics Core and the Flow Cytometry/BL3 Core. The mission of the combined Genomics & Proteomics & BL3-FACS Core is to provide the technical support and services for research faculty, graduate students and research support staff to perform innovative research on genes and proteins with special emphasis on the support of research in the areas of women health, HIV-AIDS, and related fields. The Genomics and Proteomics and BL3-FACS) Core is multi-functional since this Core provides the equipment and technical expertise to perform experiments on gene and protein expression and/or using flow cytometry, the computational resources for data analysis and data sharing (i.e., bioinformatics) and access to existing literature and databases to facilitate experimental design. The specific functions of this Core at Meharry are: 1) to provide to the research faculty, graduate students and research support staff of Meharry Medical College the necessary expert personnel and equipment to provide the services of automated DNA sequencing, DNA microarray analysis, real-time PCR, BL3 access and flow cytometry; 2) to stock an inventory of reagents for research and facilitate the purchase of molecular biological reagents; 3) to provide technical assistance and training in the use of shared equipment housed in the facility; 4) to provide the technical expertise, hardware and software for the analysis of genomic and proteomic data; and 5) to provide a program of training on topics such as genomics and proteomics (and use of instruments such as FACS) for faculty, staff, graduate students and post-doctoral fellows. The ultimate goal of the Core is to provide services needed by the research community at Meharry to increase research productivity as evidenced by increased faculty grant submissions and acquisitions, and increased publication numbers by faculty. In summary, the primary purpose of the Genomics and Proteomics and BL3-FACS Core is to provide on a continuing basis services to enhance the capability of the researchers at Meharry Medical College to perform significant biomedical research. The major service activities of the Genomics and Proteomics and BL3-FACS) Core are to provide accurate DNA sequence information rapidly and cost-effectively, access to FACS analysis and BL3 level biological containment, provide technical assistance with the use of shared equipment housed in the Core, and provide convenient access to molecular biological reagents. The specific goals for this component are: 1. To provide to the faculty, students and staff of Meharry Medical College the necessary expert personnel and equipment to provide the services of automated DNA sequencing, DNA microarray analysis, real-time PCR, BL3 access and flow cytometry. 2. To provide technical assistance with the use of shared equipment housed in the Genomics and Proteomics and BL3-FACS Core. 3. To provide the technical expertise, hardware and software for the experimental design and analysis of genomic and proteomic data. 4. To provide a program of training in genomics and proteomics and the utility of flow cytometry for faculty, staff and students.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 注意：将表和数字上传到程序摘要。 A.具体目标 基因组学和蛋白质组学以及BL3流细胞仪（FACS）核心是由管理上结合基因组学和蛋白质组学核心以及流式细胞仪/BL3核心引起的。合并的基因组学和蛋白质组学和BL3-FACS核心的使命是为研究教师，研究生和研究支持人员提供技术支持和服务，以对基因和蛋白质进行创新研究，并特别强调在女性健康，HIV-AID和相关领域的研究中支持研究。 基因组学和蛋白质组学以及BL3-FACS）核心是多功能的，因为该核心提供了对基因和蛋白质表达和/或使用流式细胞术进行实验的设备和技术专业知识，该计算资源是数据分析和数据共享（即生物信息学）的计算资源（即对现有文学和数据库访问实验性设计）。梅哈里（Meharry）的该核心的具体功能是：1）向梅哈里医学院（Meharry Medical College）的研究教师，研究生和研究支持人员提供必要的专家人员和设备，以提供自动DNA测序，DNA微阵列分析，实时PCR，BL3访问和流式细胞仪的服务； 2）库存研究库存，以供研究并促进购买分子生物试剂； 3）提供技术援助和培训，以使用设施中的共享设备； 4）提供技术专长，硬件和软件，以分析基因组和蛋白质组学数据； 5）为教师，教职员工，研究生和博士后研究员提供有关基因组学和蛋白质组学（以及诸如FACS之类的仪器）等主题的培训计划。核心的最终目标是提供梅哈里研究界所需的服务，以提高研究生产力，这可以通过增加的教师赠款提交和收购，并增加了教师的发表人数。 总而言之，基因组学和蛋白质组学和BL3-FACS核心的主要目的是提供持续的基础服务，以增强Meharry医学院的研究人员进行重要的生物医学研究的能力。基因组学和蛋白质组学和BL3-FACS的主要服务活性是快速和成本效率地提供准确的DNA序列信息，访问FACS分析和BL3水平的生物遏制，并在核心中构成的共享设备的使用中提供技术帮助，并提供方便地访问分子生物学探测器。该组件的具体目标是： 1。为了提供梅哈里医学院的学生和教职员工，提供必要的专家人员和设备，以提供自动DNA测序，DNA微阵列分析，实时PCR，BL3访问和流式细胞仪的服务。 2。在使用基因组学和蛋白质组学和BL3-FACS核心的共享设备方面提供技术帮助。 3。为基因组和蛋白质组学数据的实验设计和分析提供技术专长，硬件和软件。 4。提供一项基因组学和蛋白质组学培训计划以及教职员工和学生流式细胞仪的实用性。