Bioinformatics and Biotechnology

通过定制阵列实验室。第二个实验室还支持进口和开发 与当前提案特别相关的新阵列技术，例如 micro-RNA 阵列。的 Custom Array 实验室已运行 7 年，迄今为止已处理了 5,500 多个样本。 Core B 不断投资于最先进的仪器：最近的增强功能包括新的 点阵打印机 (Qarray2) 和几个新的 Affymetrix 流体工作站和扫描仪（总共 8 个） 流体工作站和 3 个高分辨率扫描仪，每个扫描仪均配有自动进样器）。这些实验室共同 制作并协助分析了超过 27,000 个微阵列图谱，从而做出了重要贡献 参与本项目和 SJCRH 的其他实验室的研究。核心B还包括大量 生物信息学和生物统计学专业知识将为 计划。这些对于完成基于阵列的实验至关重要的资源包括八个 博士级生物信息学家（其中一位致力于此P01，曲春旭博士）和其他12位高度 熟练的技术人员。博士。生物统计学教员庞兹和赵也带来了独特的 以及微阵列数据分析方面丰富的专业知识。与相关的高性能计算设施 Core B 包括 420 个 CPU 的 Linux 集群、150 Tb 存储容量和丰富的生物信息学 应用程序包括 LaserGene、Affymetrix LIMS 系统、Spotfire DecisionSite、R 和许多其他 适用于序列和基因表达分析的应用。我们寻求对其中一项的部分支持 致力于该计划的生物信息学家（屈春旭博士）。 相关性（参见说明）： 为儿童脑肿瘤的研究提供分子谱分析和生物信息学支持 项目/

through the Custom Array laboratory. This second laboratory also supports the import and development of new array technologies e.g., micro-RNA arrays, that are of particular relevance to the current proposal. The Custom Array laboratory has been in operation for 7 years, and has processed over 5,500 samples to date. Core B is investing continually in state-of-the-art instrumentation: recent enhancements include a new spotted array printer (Qarray2) and several new Affymetrix fluidics workstations and scanners (in total 8 fluidics workstations and 3 high-resolution scanners each with autosamplers). Together, these laboratories have produced and assisted in the analysis of over 27,000 microarray profiles, thereby contributing critically to the research of this Program and other laboratories across SJCRH. Core B also includes substantial bioinformatics and biostatistics expertise that will provide support and computational hardware to the Program. These resources that are crucial for the completion of array based experiments include eight Ph.D.-level bioinformaticists (one of which is dedicated to this P01, Dr. Chunxu Qu) and 12 other highly skilled technical staff. Drs. Pounds and Zhao, who are faculty members in Biostatistics, also bring unique and extensive expertise in microarray data analysis. The high performance computing facility associated with Core B includes a 420-cpu Linux cluster, 150 Tb storage capacity, and a wealth of bioinformatics applications including LaserGene, Affymetrix LIMS system, Spotfire DecisionSite, R, and many other applications suitable for sequence and gene expression analysis. We seek partial support for one bioinformaticist dedicated to this Program (Dr. Chunxu Qu). RELEVANCE (See instructions): To provide molecular profiling and bioinformatics support for investigation of pediatric brain tumors PROJECT/