BIACORE 3000

生物传感器技术现在是实时监测生物分子相互作用动力学的选择方法。该技术具有很高的灵敏度、特异度和重现性，需要极少量的材料，适合于研究各种生物分子之间的相互作用，包括小有机分子、蛋白质、核酸、碳水化合物和脂类。广泛的文献展示了在药物/受体相互作用、信号转导、转录、膜蛋白和免疫调节研究中的应用。拥有测量这些类型的相互作用的能力是SJCRH正在进行的五个主要研究项目的关键，但我们无论是在该机构还是在该市都没有这种能力。这项申请寻求购买生物传感器仪器Biacore 3000的资金，以支持NIH资助的这些研究工作。该仪器将并入圣犹大儿童研究医院的生物技术中心。

Biosensor technology is now the method of choice to monitor in real-time the kinetics of biomolecular interactions. The technology offers high sensitivity, specificity and reproducibility, requires minute amounts of material, and is appropriate to the study of interactions between a wide range of biomolecules, including small organic molecules, proteins, nucleic acids, carbohydrates, and lipids. A broad literature demonstrates applications in studies of drug/receptor interactions, signal transduction, transcription, membrane proteins and immune regulation. Having the ability to measure these types of interactions are essential to five major research programs underway at SJCRH, yet we do not have this capability either in the institution or in the city. This application seeks funding for the purchase of a biosensor instrument, the Biacore 3000, to support these NIH-funded research efforts. The instrument will be incorporated into the Center for Biotechnology at St. Jude Children's Research Hospital.