Bioinformatics and Biotechnology

通过定制阵列实验室第二个实验室还支持进口和开发 新的阵列技术例如，微RNA阵列，这是特别相关的目前的建议。的 自定义阵列实验室已经运行了7年，迄今已处理了5，500多个样本。 核心B不断投资于最先进的仪器：最近的改进包括一个新的 点阵列打印机（Qarray 2）和几个新的Affyphon射流工作站和扫描仪（共8个 射流工作站和3台高分辨率扫描仪，每台扫描仪都配有自动进样器）。这些实验室 已经制作并协助分析了27，000多个微阵列图谱，从而为 本项目和SJCRH其他实验室的研究。核心B还包括大量 生物信息学和生物统计学专业知识，将提供支持和计算硬件， 程序.这些对于完成基于阵列的实验至关重要的资源包括八个 博士学位-级生物信息学家（其中一位致力于本P01，曲春旭博士）和其他12位高级生物信息学家 熟练的技术人员。Pounds和Zhao博士是生物统计学的教员，他们也带来了独特的 以及在微阵列数据分析方面的广泛专业知识。高性能计算设施与 核心B包括一个420 CPU的Linux集群、150 Tb的存储容量和丰富的生物信息学 应用程序包括LaserGene、Affytechnic LIMS系统、Spotfire DecisionSite、R和许多其他 适用于序列和基因表达分析的应用。我们寻求部分支持， 致力于该项目的生物信息学家（曲春旭博士）。 相关性（参见说明）： 为儿童脑肿瘤的研究提供分子图谱和生物信息学支持 项目/

through the Custom Array laboratory. This second laboratory also supports the import and development of new array technologies e.g., micro-RNA arrays, that are of particular relevance to the current proposal. The Custom Array laboratory has been in operation for 7 years, and has processed over 5,500 samples to date. Core B is investing continually in state-of-the-art instrumentation: recent enhancements include a new spotted array printer (Qarray2) and several new Affymetrix fluidics workstations and scanners (in total 8 fluidics workstations and 3 high-resolution scanners each with autosamplers). Together, these laboratories have produced and assisted in the analysis of over 27,000 microarray profiles, thereby contributing critically to the research of this Program and other laboratories across SJCRH. Core B also includes substantial bioinformatics and biostatistics expertise that will provide support and computational hardware to the Program. These resources that are crucial for the completion of array based experiments include eight Ph.D.-level bioinformaticists (one of which is dedicated to this P01, Dr. Chunxu Qu) and 12 other highly skilled technical staff. Drs. Pounds and Zhao, who are faculty members in Biostatistics, also bring unique and extensive expertise in microarray data analysis. The high performance computing facility associated with Core B includes a 420-cpu Linux cluster, 150 Tb storage capacity, and a wealth of bioinformatics applications including LaserGene, Affymetrix LIMS system, Spotfire DecisionSite, R, and many other applications suitable for sequence and gene expression analysis. We seek partial support for one bioinformaticist dedicated to this Program (Dr. Chunxu Qu). RELEVANCE (See instructions): To provide molecular profiling and bioinformatics support for investigation of pediatric brain tumors PROJECT/