ANIMAL CORE

动物核心

基本信息

批准号：
8167928
负责人：
William L. Woolverton
金额：
$ 25.49万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8167928
关键词：
Address Adult Affect American Animal Experimentation Animals Antidepressive Agents Autopsy Behavioral Behavioral Symptoms Blood specimen Brain Caring Chronic Chronic stress Collection Computer Retrieval of Information on Scientific Projects Database Disease Dissection Doctor of Philosophy Ethics Faculty Fluoxetine Freezing Funding Glutamates Grant High Pressure Liquid Chromatography Human Individual Institution Lead Life Long-Term Effects Medical Medical center Mental Depression Mental disorders Mississippi Moods Neuraxis Neurosciences Pharmaceutical Preparations Process Research Research Personnel Resources Sampling Serotonin Source Supervision System Thinking Training United States National Institutes of Health Universities analytical method brain tissue effective therapy experience high standard liquid chromatography mass spectrometry method development neurochemistry novel physical conditioning restraint stress sample fixation

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Center for Psychiatric Neuroscience (CPN) examines the neurochemical causes of depression. Of all mental illnesses, depression is the most common disorder and is a serious and persistent medical illness affecting the thoughts, mood, activity, and physical health of 9.9 million American adults in any given year. Integrated studies conducted by CPN investigators use postmortem brain tissue from animals treated chronically with an antidepressant drug or exposed to chronic stress to examine novel hypotheses that may ultimately lead to more effective treatments for this debilitating disease. The CPN and the University of Mississippi Medical Center (UMC) use research animals when the information that will be gained may be useful in saving human lives or treating disease and/or when the information cannot be gained without the use of animals. The CPN and UMC recognize its ethical responsibility to treat animals involved in research humanely, and require that all faculty and staff involved in animal research undergo vigorous training and maintain the highest standards of care. The CPN Animal Core provides brain tissue from animal subjects that have been treated either pharmacologically or behaviorally in a manner that allows CPN investigators to address the specific aims of their individual projects. To allow the assessment of the effects of long-term antidepressant exposure on central nervous system parameters, animals are treated long-term with fluoxetine and sacrificed for postmortem studies of changes in the brain. In addition to the studies with fluoxetine, other animals are exposed to a chronic stress paradigm that results in behavioral symptoms that may be related to human depression. Chronic restraint stress results in alterations in some features of the glutamate and serotonin systems that may well be relevant to the changes detected in these systems in human depression. William L. Woolverton, Ph.D., provides overall supervision of the Animal Core. He is a behavioral pharmacologist with 30 years experience with pharmacological research in behavioral and neurochemical studies. Javier Miguel-Hidalgo, Ph.D., supervises brain dissection, collection and storage. Dr. Miguel-Hidalgo is a neuroanatomist with 18 years experience in dissection, freezing, fixation, storage, and histochemical processing of brain samples in a variety of vertebrate species. Patrick Kyle, Ph.D., will assess fluoxetine levels in blood samples. Dr. Kyle is an analytical toxicologist with 14 years experience in drug analysis and analytical methods development involving GC/MS, HPLC, and LC/MS.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。精神神经科学中心（CPN）研究了抑郁症的神经化学原因。在所有精神疾病中，抑郁症是最常见的疾病，是一种严重且持续的医学疾病，影响到任何给定年份的990万美国成年人的思想，情绪，活动和身体健康。 CPN研究人员进行的综合研究使用了用抗抑郁药或暴露于慢性应激的动物的死后脑组织，以检查新的假设，这些假设最终可能导致对这种衰减疾病的更有效治疗。 CPN和密西西比大学医学中心（UMC）使用研究动物时，将获得的信息可能有助于挽救人类的生命或治疗疾病和/或在没有动物的情况下获得信息。 CPN和UMC认识到其人道研究的动物的道德责任，并要求所有参与动物研究的教职员工都接受了剧烈的培训并保持最高的护理标准。 CPN动物核心提供来自动物受试者的脑组织，这些动物受试者在药理学或行为上以允许CPN研究人员来解决其各个项目的特定目的的方式。为了评估长期抗抑郁药对中枢神经系统参数的影响，用氟西汀对动物进行了长期处理，并为大脑变化的死后研究处死。除了对氟西汀的研究外，其他动物还暴露于慢性应激范式中，导致行为症状可能与人类抑郁症有关。慢性克制压力导致谷氨酸和5-羟色胺系统的某些特征的改变，这可能与人类抑郁症中这些系统中检测到的变化有关。威廉·L·伍尔弗顿（William L. Woolverton）博士提供了对动物核心的整体监督。他是一名行为药物学家，在行为和神经化学研究方面具有30年的药理研究经验。哈维尔·米格尔·希达尔戈（Javier Miguel-Hidalgo）博士监督大脑解剖，收集和存储。 Miguel-Hidalgo博士是一位神经解剖学家，在各种脊椎动物物种中对脑样品的解剖，冻结，固定，储存和组织化学处理有18年的经验。帕特里克·凯尔（Patrick Kyle）博士将评估血液样本中的氟西汀水平。 Kyle博士是一位分析毒理学家，在涉及GC/MS，HPLC和LC/MS的药物分析和分析方法开发方面具有14年的经验。