ANIMAL CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 精神神经科学中心(CPN)研究抑郁症的神经化学原因。在所有精神疾病中，抑郁症是最常见的疾病，是一种严重而持久的疾病，每年影响990万美国成年人的思想、情绪、活动和身体健康。CPN调查人员进行的综合研究使用了长期使用抗抑郁药物治疗或暴露在慢性压力下的动物的死后脑组织，以检验新的假设，这些假设最终可能导致对这种衰弱疾病进行更有效的治疗。 CPN和密西西比大学医学中心(UMC)使用研究动物，当所获得的信息可能对拯救人类生命或治疗疾病有用，和/或如果不使用动物就无法获得这些信息。CPN和UMC认识到以人道方式对待参与研究的动物的道德责任，并要求所有参与动物研究的教职员工接受严格的培训，并保持最高的护理标准。 CPN动物核心提供来自动物受试者的脑组织，这些受试者经过药物治疗或行为治疗，使CPN调查人员能够解决其个别项目的具体目标。为了评估长期接触抗抑郁剂对中枢神经系统参数的影响，动物被长期用氟西汀治疗，并被处死以进行大脑变化的尸检研究。 除了对氟西汀的研究外，其他动物还暴露在慢性应激模式下，这种模式会导致可能与人类抑郁有关的行为症状。慢性束缚应激导致谷氨酸和5-羟色胺系统的某些特征发生变化，这可能与在人类抑郁症中检测到的这些系统的变化有关。 威廉·L·伍尔弗顿博士负责动物核心的全面监督。他是一名行为药理学家，在行为和神经化学研究方面有30年的药理学研究经验。 哈维尔·米格尔-伊达尔戈博士负责监督大脑解剖、收集和存储。米格尔-伊达尔戈博士是一位神经解剖学家，在各种脊椎动物脑组织样本的解剖、冷冻、固定、储存和组织化学处理方面拥有18年的经验。 帕特里克·凯尔博士将评估血液样本中的氟西汀水平。凯尔博士是一名分析毒物学家，在药物分析和分析方法开发方面拥有14年的经验，包括GC/MS、HPLC和LC/MS。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Center for Psychiatric Neuroscience (CPN) examines the neurochemical causes of depression. Of all mental illnesses, depression is the most common disorder and is a serious and persistent medical illness affecting the thoughts, mood, activity, and physical health of 9.9 million American adults in any given year. Integrated studies conducted by CPN investigators use postmortem brain tissue from animals treated chronically with an antidepressant drug or exposed to chronic stress to examine novel hypotheses that may ultimately lead to more effective treatments for this debilitating disease. The CPN and the University of Mississippi Medical Center (UMC) use research animals when the information that will be gained may be useful in saving human lives or treating disease and/or when the information cannot be gained without the use of animals. The CPN and UMC recognize its ethical responsibility to treat animals involved in research humanely, and require that all faculty and staff involved in animal research undergo vigorous training and maintain the highest standards of care. The CPN Animal Core provides brain tissue from animal subjects that have been treated either pharmacologically or behaviorally in a manner that allows CPN investigators to address the specific aims of their individual projects. To allow the assessment of the effects of long-term antidepressant exposure on central nervous system parameters, animals are treated long-term with fluoxetine and sacrificed for postmortem studies of changes in the brain. In addition to the studies with fluoxetine, other animals are exposed to a chronic stress paradigm that results in behavioral symptoms that may be related to human depression. Chronic restraint stress results in alterations in some features of the glutamate and serotonin systems that may well be relevant to the changes detected in these systems in human depression. William L. Woolverton, Ph.D., provides overall supervision of the Animal Core. He is a behavioral pharmacologist with 30 years experience with pharmacological research in behavioral and neurochemical studies. Javier Miguel-Hidalgo, Ph.D., supervises brain dissection, collection and storage. Dr. Miguel-Hidalgo is a neuroanatomist with 18 years experience in dissection, freezing, fixation, storage, and histochemical processing of brain samples in a variety of vertebrate species. Patrick Kyle, Ph.D., will assess fluoxetine levels in blood samples. Dr. Kyle is an analytical toxicologist with 14 years experience in drug analysis and analytical methods development involving GC/MS, HPLC, and LC/MS.