ANIMAL CORE

动物核心

• 批准号: 8360502
• 负责人: William L. Woolverton
• 金额: $ 12.19万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360502
• 关键词: Address; Adult; Affect; American; Animal Experimentation; Animals; Antidepressive Agents; Autopsy; Behavioral; Behavioral Symptoms; Blood specimen; Brain; Caring; Chronic; Chronic stress; Collection; Disease; Dissection; Doctor of Philosophy; Ethics; Faculty; Fluoxetine; Freezing; Funding; Glutamates; Grant; High Pressure Liquid Chromatography; Human; Individual; Lead; Long-Term Effects; Medical; Medical center; Mental Depression; Mental disorders; Mississippi; Moods; National Center for Research Resources; Neuraxis; Neurosciences; Pharmaceutical Preparations; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Serotonin; Source; Supervision; System; Thinking; Training; United States National Institutes of Health; Universities; analytical method; brain tissue; cost; effective therapy; experience; high standard; liquid chromatography mass spectrometry; method development; neurochemistry; novel; physical conditioning; restraint stress; sample fixation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Center for Psychiatric Neuroscience (CPN) examines the neurochemical causes of depression. Of all mental illnesses, depression is the most common disorder and is a serious and persistent medical illness affecting the thoughts, mood, activity, and physical health of 9.9 million American adults in any given year. Integrated studies conducted by CPN investigators use postmortem brain tissue from animals treated chronically with an antidepressant drug or exposed to chronic stress to examine novel hypotheses that may ultimately lead to more effective treatments for this debilitating disease. The CPN and the University of Mississippi Medical Center (UMC) use research animals when the information that will be gained may be useful in saving human lives or treating disease and/or when the information cannot be gained without the use of animals. The CPN and UMC recognize its ethical responsibility to treat animals involved in research humanely, and require that all faculty and staff involved in animal research undergo vigorous training and maintain the highest standards of care. The CPN Animal Core provides brain tissue from animal subjects that have been treated either pharmacologically or behaviorally in a manner that allows CPN investigators to address the specific aims of their individual projects. To allow the assessment of the effects of long-term antidepressant exposure on central nervous system parameters, animals are treated long-term with fluoxetine and sacrificed for postmortem studies of changes in the brain. In addition to the studies with fluoxetine, other animals are exposed to a chronic stress paradigm that results in behavioral symptoms that may be related to human depression. Chronic restraint stress results in alterations in some features of the glutamate and serotonin systems that may well be relevant to the changes detected in these systems in human depression. William L. Woolverton, Ph.D., provides overall supervision of the Animal Core. He is a behavioral pharmacologist with 30 years experience with pharmacological research in behavioral and neurochemical studies. Javier Miguel-Hidalgo, Ph.D., supervises brain dissection, collection and storage. Dr. Miguel-Hidalgo is a neuroanatomist with 18 years experience in dissection, freezing, fixation, storage, and histochemical processing of brain samples in a variety of vertebrate species. Patrick Kyle, Ph.D., will assess fluoxetine levels in blood samples. Dr. Kyle is an analytical toxicologist with 14 years experience in drug analysis and analytical methods development involving GC/MS, HPLC, and LC/MS.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 精神神经科学中心（CPN）研究抑郁症的神经化学原因。在所有精神疾病中，抑郁症是最常见的疾病，是一种严重和持续的医学疾病，在任何一年都会影响990万美国成年人的思想，情绪，活动和身体健康。CPN研究人员进行的综合研究使用了长期接受抗抑郁药物治疗或暴露于慢性应激的动物的死后脑组织，以研究可能最终导致这种衰弱疾病更有效治疗的新假设。 CPN和密西西比大学医学中心（UMC）使用研究动物时，将获得的信息可能是有用的，在挽救人类生命或治疗疾病和/或当信息不能获得不使用动物。CPN和UMC认识到其人道对待参与研究的动物的道德责任，并要求所有参与动物研究的教职员工接受严格的培训，并保持最高的护理标准。 CPN Animal Core提供了来自动物受试者的脑组织，这些动物受试者以一种允许CPN调查人员解决其个人项目的具体目标的方式接受了脑组织或行为治疗。为了评估长期抗抑郁药暴露对中枢神经系统参数的影响，用氟西汀长期治疗动物，并处死动物进行脑变化的死后研究。 除了氟西汀的研究外，其他动物也暴露于慢性应激范式，导致可能与人类抑郁症相关的行为症状。慢性束缚应激导致谷氨酸和5-羟色胺系统某些特征的改变，这可能与人类抑郁症中这些系统中检测到的变化有关。 William L.伍尔弗顿博士，对动物中心进行全面监督。他是一名行为药理学家，在行为和神经化学研究方面拥有30年的药理学研究经验。 Javier Miguel-Hidalgo博士，负责大脑的解剖收集和储存 Miguel-Hidalgo博士是一位神经解剖学家，在解剖、冷冻、固定、储存和组织化学处理各种脊椎动物大脑样本方面拥有18年的经验。 帕特里克凯尔博士，将评估血液样本中的百忧解水平。凯尔博士是一名分析毒理学家，在药物分析和分析方法开发（包括GC/MS、HPLC和LC/MS）方面拥有14年经验。