Flow Cytometry

流式细胞仪

• 批准号: 8181120
• 负责人: Marty Bigos
• 金额: $ 8.8万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8181120
• 关键词: Adopted; Area; Arts; Biological; Budgets; Cancer Center; Cell Separation; Cells; Clinical Trials; Cloning; Communities; Consultations; Data Analyses; Development; Equipment; Experimental Designs; Faculty; Financial compensation; Flow Cytometry; Flow Cytometry Shared Resource; Future; Goals; Hour; Laboratories; Maintenance; Malignant Neoplasms; Measurement; Mission; Patients; Phosphorylation; Proteins; Quality Control; Research; Research Personnel; Research Training; Resource Sharing; Resources; Services; Signal Transduction; Solid Neoplasm; Sorting - Cell Movement; Techniques; Technology; Time; Training; Universities; Work; cell analyzer; cost effective; experience; human stem cells; humanized antibody; instrument; instrumentation; medical schools; member; new technology; operation; programs; tool

The mission of the Flow Cytometry Shared Resource (also known as FACS) Facility is to provide state-of-the-art highly available flow cytometry and cell-sorting services to the Stanford research community. To fulfill this mission, the Facility maintains three cell sorters and four flow analyzers in the Beckman Center in the School of Medicine. Four full-time and seven part-time staff work in the Facility, providing quality control, maintenance and operation of these instruments, as well as user training and consultation on experimental design and data analysis. The staff embodies over 125 years of flow cytometry experience, including developing some of the major advances of the field, such as compensation, cloning and large numbers of measurement parameters. The Facility Faculty Advisor is also highly experienced with flow cytometry, having used it as a research tool and helped to develop new applications over a 15-year period. The Flow Cytometry Shared Resource Facility billed 10,094 hours for instrument use in 2008. This represented 389 users from 153 different laboratories at Stanford. Cancer Center members provide the majority of this use, representing 117 of the laboratories and logging 76% of the hours. The FY 2008-2009 budget is over $900,000 and is expected to grow as new instrumentation is added. Currently, instruments with greater capabilities are utilized at close to 100% capacity. To this end, a new high-end analyzer and cell sorter have recently been obtained. The future goals of the Facility include expanding its educational program and bringing in new technologies and instrumentation, in addition to continuing to providing the highest quality flow cytometry services to the Cancer Center.

流式细胞仪共享资源（也称为FACS）设施的使命是为斯坦福大学研究社区提供最先进的高度可用的流式细胞仪和细胞分选服务。为了完成这一使命，该设施在医学院贝克曼中心拥有三台细胞分选仪和四台流动分析仪。该设施有四名全职和七名兼职工作人员，负责这些仪器的质量控制、维护和操作，以及用户培训和咨询， 实验设计和数据分析。我们的员工拥有超过125年的流式细胞术经验，包括开发该领域的一些重大进展，如补偿，克隆和大量测量参数。该设施的教师顾问也对流式细胞术有着丰富的经验，将其用作研究工具，并在15年的时间内帮助开发新的应用。 2008年，流式细胞术共享资源设施为仪器使用收取了10，094小时的费用。这代表了来自斯坦福大学153个不同实验室的389名用户。癌症中心的成员提供了大部分的这种使用，代表117个实验室和记录76%的小时。2008-2009财年的预算超过90万美元，预计将随着新仪器的增加而增长。目前，具有更大能力的仪器的利用率接近100%。为此，一种新的高端分析仪和 最近已经获得了细胞分选器。 该设施的未来目标包括扩大其教育计划，引进新技术和仪器，以及继续为癌症中心提供最高质量的流式细胞术服务。