LSR-II FLOW CYTOMETER: MOLECULAR MECHANISMS OF HIV

点击翻译按钮获取中文摘要

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): Support is requested to pay off a lease-to-purchase of a Becton Dickenson Custom LSR-II flow cytometer, and to add an HTS to the LSR-II as well as a high-end Apple workstation for data analysis. A group of six major users with eleven NIH funded projects centering on a broad range of HIV research used an LSR-II that the Gladstone Flow Core shared with a UCSF Core Immunology Laboratory. In late 2004, the Gladstone Institute relocated to a new site; access to this LSR-II decreased due to remoteness and increased use by the CIL. The requested instrument was leased so the major user group could effectively continue their projects. The LSR-II requested is a custom instrument with extra lasers and PMTs. Measurements made with other cytometers indicate that this configuration yields superior measurements on many parameters. The HTS would enhance at least one project of each of the major users. The Apple workstation will increase the availability of high-end data analysis using FlowJo for the major users.