Flow Cytometry

流式细胞仪共享资源（FCSR）是一个尖端的设施，为SCI会员提供细胞分析和细胞分选服务。共享资源的使命是促进最高质量和严格水平的科学研究和培训，并将细胞计数仪器、技术和分析的新发展纳入生物用途和日常可用性。FCSR通过促进高内容的数据采集，确保最高的数据质量和一致性，进行高效的细胞分选，提供可靠的数据管理，教育和培训研究人员以及在实验设计和评估方面提供咨询，提高研究生产力和有效性。流式细胞术服务的主要应用包括：肿瘤生物学的DNA含量分析、淋巴瘤细胞的免疫学表征、干细胞和免疫细胞分选、肿瘤中关键细胞群的分离以及用于肿瘤分类的细胞信号级联的定义。FCSR实现其双重使命，为研究人员提供高效和具有成本效益的设备，专业知识和尖端技术，以满足他们的细胞分析和分选需求，以及领导或支持新的流动技术和应用的开发。共享资源的运营预算为140万美元。Marty Bigos是流式细胞术共享资源的主任，Garry Nolan博士是学院顾问。未来的计划包括扩大新的质谱分析服务。

The Flow Cytometry Shared Resource (FCSR) is a cutting-edge facility that provides cell analysis and cell sorting services for SCI members. The mission of the shared resource is to promote scientific research and training with the highest level of quality and rigor and to bring new developments in cytometric instrumentation, techniques and analysis into biological use and routine availability. The FCSR enhances research productivity and effectiveness by promoting high-content data acquisition, ensuring the highest data quality and consistency, carrying out efficient cell sorting, providing reliable data management, educating and training researchers and consulting in experiment design and evaluation. Major applications of flow cytometry services include: DNA content analysis for tumor biology, immunological characterization of lymphoma cells, stem and immune cell sorting, isolation of key cell population in tumors and definition of cell signaling cascades for tumor classification. The FCSR achieves its twofold mission by providing investigators efficient and cost-effective access to equipment, expertise and cutting-edge techniques for their cell analysis and sorting needs, as well as leading or supporting the development of new flow technologies and applications. The operating budget of the Shared Resource is $1.4 M. Marty Bigos is the Director of the Flow Cytometry Shared Resource and Garry Nolan, PhD, is the Faculty Advisor. Future plans include expanding the new Mass Cytometry services.