LSR-II FLOW CYTOMETER: HTLV & LEUKEMIA

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：请提供支持，以支付购买Becton Dickenson Custom LSR-II流式细胞仪的租赁费用，并为LSR-II增加HTS以及用于数据分析的高端Apple工作站。一个由六个主要用户组成的小组，有11个NIH资助的项目，以广泛的艾滋病毒研究为中心，使用了Gladstone Flow Core与加州大学旧金山分校的核心免疫学实验室共享的LSR-II。2004年底，格拉德斯通研究所搬迁到一个新的地点；由于地处偏远和CIL增加了使用，进入该LSR-II的机会减少了。租用了所要求的仪器，以便主要用户群体能够有效地继续他们的项目。所要求的LSR-II是一个带有额外激光器和PMT的定制仪器。用其他细胞仪进行的测量表明，这种配置在许多参数上都能产生更好的测量结果。HTS将增强每个主要用户的至少一个项目。Apple工作站将为主要用户增加使用FlowJo进行高端数据分析的可用性。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Support is requested to pay off a lease-to-purchase of a Becton Dickenson Custom LSR-II flow cytometer, and to add an HTS to the LSR-II as well as a high-end Apple workstation for data analysis. A group of six major users with eleven NIH funded projects centering on a broad range of HIV research used an LSR-II that the Gladstone Flow Core shared with a UCSF Core Immunology Laboratory. In late 2004, the Gladstone Institute relocated to a new site; access to this LSR-II decreased due to remoteness and increased use by the CIL. The requested instrument was leased so the major user group could effectively continue their projects. The LSR-II requested is a custom instrument with extra lasers and PMTs. Measurements made with other cytometers indicate that this configuration yields superior measurements on many parameters. The HTS would enhance at least one project of each of the major users. The Apple workstation will increase the availability of high-end data analysis using FlowJo for the major users.