LSR-II FLOW CYTOMETER: HTLV & LEUKEMIA

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：请求支持支付一台Becton Dickenson Custom LSR-II流式细胞仪的租赁购买，并在LSR-II上增加一台HTS和一台高端Apple工作站用于数据分析。一组6个主要用户与11个NIH资助的项目集中在广泛的艾滋病毒研究中，使用LSR-II， Gladstone Flow Core与UCSF核心免疫学实验室共享。2004年底，格莱斯顿研究所搬迁到新址；由于地处偏远和CIL的使用增加，对该LSR-II的访问减少了。租用了所要求的仪器，以便主要用户组能够有效地继续其项目。要求的LSR-II是带有额外激光器和pmt的定制仪器。用其他细胞仪进行的测量表明，这种配置在许多参数上产生优越的测量结果。高通量运输系统将加强每个主要用户的至少一个项目。苹果工作站将为主要用户增加使用FlowJo进行高端数据分析的可用性。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Support is requested to pay off a lease-to-purchase of a Becton Dickenson Custom LSR-II flow cytometer, and to add an HTS to the LSR-II as well as a high-end Apple workstation for data analysis. A group of six major users with eleven NIH funded projects centering on a broad range of HIV research used an LSR-II that the Gladstone Flow Core shared with a UCSF Core Immunology Laboratory. In late 2004, the Gladstone Institute relocated to a new site; access to this LSR-II decreased due to remoteness and increased use by the CIL. The requested instrument was leased so the major user group could effectively continue their projects. The LSR-II requested is a custom instrument with extra lasers and PMTs. Measurements made with other cytometers indicate that this configuration yields superior measurements on many parameters. The HTS would enhance at least one project of each of the major users. The Apple workstation will increase the availability of high-end data analysis using FlowJo for the major users.