Animal Shared Resource

动物共享资源（ASR）的功能是促进有效、经济和最先进的动物模型的使用，以进行癌症相关研究。这是通过一个集中的资源，其中AALAS认证的兽医技术人员/技术人员提供高质量的研究服务，伦巴第成员完成。一个重要的重点是使用转基因小鼠，无胸腺小鼠， 还有斑马鱼提供的主要服务包括协调动物和特殊动物饲料购买要求;执行特定操作，包括饮食，激素或药物管理，以改变肿瘤膨胀，促进，进展或转移;接种肿瘤细胞或肿瘤细胞系在动物体内繁殖;给予致癌物;监测肿瘤生长;收集肿瘤，器官和血清;这些研究包括：进行尸检和活组织检查;以及斑马鱼研究，包括用反义吗啉代寡核苷酸（MO）显微注射卵以敲低感兴趣的特定基因;用RNA或cDNA显微注射卵以过表达野生型、突变型和嵌合基因;筛选毒性/药物;产生转基因斑马鱼;移植细胞;以及异种移植实验。ASR位于乔治敦大学医学院比较医学部（DCM）。DCM是一个集中的、AAALAC认证的、USDA注册的动物机构，并在NIH存档了一份批准的保证书。作为综合健康监测计划的一部分，通过在每个动物房的每个架子上饲养哨兵小鼠来监测一般环境和动物健康。所有免疫缺陷 啮齿类动物保持在无特定病原体的环境中。大多数转基因小鼠群体被维持在专用屏障隔离设施中。该资源的服务对于Lombardi研究人员至关重要，他们进行临床前研究，使用最先进的，适当的，有效的和经济的动物模型来测试与癌症治疗和预防相关的假设。训练有素、经验丰富的技术人员与Lombardi成员密切合作，提供高质量的动物模型技术服务，以支持需要使用动物的同行评审癌症研究。2008年，这些服务被用于支持18名Lombardi成员在五个癌症中心方案中的同行评审项目。

The function of Animal Shared Resource (ASR) is to facilitate the efficient, economical, and state-of-art use of animal models for the performance of cancer-related studies. This is accomplished through a centralized resource, where AALAS-certified veterinary technicians/technologists provide high-quality research services to Lombardi members. A significant emphasis is placed on the use of genetically modified mice, athymic mice, and zebrafish. Major services provided include coordination of animal and special animal feed purchase requests; performance of specific manipulations, including diet, hormone or drug administrations to modify tumor inflation, promotion, progression or metastasis; inoculation of tumor cells or in vivo propagation of tumor cell lines in animals; administration of carcinogens; monitoring tumor growth; collection of tumors, organs, and sera; performance of necropsies and biopsies; and zebrafish studies, including microinjecting eggs with antisense Morpholino oligonucleotides (MO) to knock down specific genes of interest; microinjecting eggs with RNA or cDNA to overexpress wild-type, mutant and chimeric genes; screening for toxicity/drugs; generating transgenic zebrafish; transplanting cells; and xenotransplantation experiments. The ASR is located within Georgetown University Medical School's Division of Comparative Medicine (DCM). The DCM is a centralized, AAALAC-accredited, USDA-registered animal facility and has an approved letter of assurance on file at the NIH. The general environment and animal health is monitored through the use of sentinel mice maintained on each rack in each animal room as part of a comprehensive health surveillance program. All immune-deficient rodents are maintained within a Specific Pathogen Free environment. Most genetically modified mice colonies are maintained in a dedicated barrier isolation facility. The services of this resource are critical for the Lombardi investigators who perform preclinical studies to test hypotheses related to treatment and prevention of cancer using state-of-art, and appropriate, effective and economical animal models. The highly trained and experienced technical staff works closely with Lombardi members to provide quality animal model technical services in support of peer reviewed cancer research requiring the use of animals. In 2008, these services were used in support of peer-reviewed projects of 18 Lombardi members in five Cancer Center Programs.