Research Support Core: Analytical Chemistry Core

分析核心是由两个实验室组成的中心资源，这两个实验室促进现代分析方法的开发和应用，以解决超级基金计划各组成部分遇到的关键问题。第一个是超级基金分析实验室，它提供一系列仪器服务，重点是质谱学、色谱学和小分子分析。第二个是劳伦斯利弗莫尔国家实验室的加速器质谱学设施，配备了两台AMS仪器，用于超痕量分析。蛋白质组分析主要在核心B(以前的核心C)中提供，尽管核心A中的仪器可用于蛋白质组学工作。分析核心为项目提供非常规工具以及服务和研究协作。这些服务包括对其他校园设施的帮助，如核磁共振(核磁共振)。核心A改进了许多分析技术，包括通过开发机器人程序、样品清理、样品制备和衍生化的方法来进行准备、吞吐量和准确性。核心A支持全球和路径选择性代谢组学技术的发展及其在假说产生和检验以及生物标记物开发方面的应用。它与这些项目合作，对危险混合物进行生物测定驱动的分级，并为超级基金的科学家提供分析化学方面的教育。LLNL组件提供了获得各种先进仪器的途径，但侧重于AMS的亚阿托摩尔(即10[-18])敏感性，以支持使用[14]C作为重同位素的项目。这项技术首次允许人类使用极低水平的示踪剂在环境现实的水平上监测危险化学品的新陈代谢。AMS的优先目标是量化人类对杀虫剂氯菊酯和个人护理产品三氯卡班的新陈代谢。核心A级科学家在分析化学、数据生成和分析方面提供一对一的协助，并继续为超级基金研究人员提供分析技术方面的正式培训。 因此，核心A为开发和使用暴露和影响的生物标志物提供了必要的广泛分析支持。

The Analytical Core is a central resource composed of two laboratories that facilitate-development and application of modern analytical methods to solve key problems encountered by the components of the Superfund Program. The first is the Superfund Analytical Laboratory which provides a range of instrument services with emphasis on mass spectrometry and chromatography and small molecule analysis. The second is the Accelerator Mass Spectrometry Facility with 2 AMS instruments at Lawrence Livermore National Laboratory for ultra trace analysis. Proteomic analysis is largely provided in Core B (formerly Core C) although the instrumentation in Core A is available for proteomic work. The Analytical Core provides walk-up instrumentation as well as services and research collaborations to the projects. The services include assistance with other campus facilities such as nuclear magnetic resonance (NMR). Core A advances many analytical technologies, including preparation, throughput and accuracy by developing robotic procedures, sample clean up, approaches to sample preparation and derivatization. Core A supports the development of both global and pathway selective metabolomic techniques and their applications to hypothesis generation and testing as well as for biomarker development. It carries out bioassay driven fractionation of hazardous mixtures, in collaboration with the projects and provides education for Superfund scientists in analytical chemistry. The LLNL component provides access to a variety of advanced instruments but focuses on the sub attomole (i.e. 10[-18]) sensitivity of AMS in support of projects using [14]C as a heavy isotopes. This technique for the first time allows human monitoring of metabolism of hazardous chemicals at environmentally realistic levels using exceptionally low levels of tracers. Priority targets for AMS are to quantify human metabolism of the insecticide permethrin and the personal care product triclocarban. Core A scientists provide one-on-one assistance in analytical chemistry, data generation and analysis and on going formal training in analytical techniques for Superfund researchers. Thus, Core A provides the broad analytical support necessary for developing and using biomarkers of exposure and effect.