Research Support Core: Analytical Chemistry Core

分析核心是由两个实验室组成的中央资源，促进现代分析方法的开发和应用，以解决超级基金计划组成部分遇到的关键问题。第一个是超级基金分析实验室，提供一系列仪器服务，重点是质谱法和色谱法以及小分子分析。第二个是劳伦斯利弗莫尔国家实验室的加速器质谱设施，配有2台AMS仪器，用于超痕量分析。蛋白质组学分析主要在核心B（以前的核心C）中提供，尽管核心A中的仪器可用于蛋白质组学工作。分析核心提供步行仪器以及服务和研究合作的项目。这些服务包括与其他校园设施，如核磁共振（NMR）的援助。核心A通过开发机器人程序、样品清理、样品制备和衍生化方法，推进了许多分析技术，包括制备、通量和准确性。核心A支持全球和途径选择性代谢组学技术的发展及其在假设生成和测试以及生物标志物开发中的应用。它与项目合作，对危险混合物进行生物测定驱动的分馏，并为超级基金科学家提供分析化学方面的教育。LLNL部分提供了使用各种先进仪器的途径，但侧重于AMS的亚阿摩尔（即10[-18]）灵敏度，以支持使用[14]C作为重同位素的项目。这项技术首次允许人类使用极低水平的示踪剂，以环境现实水平监测危险化学品的代谢。AMS的优先目标是量化杀虫剂氯菊酯和个人护理产品三氯卡班的人体代谢。核心A科学家提供一对一的援助，在分析化学，数据生成和分析，并正在进行正式的培训，在分析技术的超级基金研究人员。 因此，核心A为开发和使用暴露和效应的生物标志物提供了广泛的分析支持。

The Analytical Core is a central resource composed of two laboratories that facilitate-development and application of modern analytical methods to solve key problems encountered by the components of the Superfund Program. The first is the Superfund Analytical Laboratory which provides a range of instrument services with emphasis on mass spectrometry and chromatography and small molecule analysis. The second is the Accelerator Mass Spectrometry Facility with 2 AMS instruments at Lawrence Livermore National Laboratory for ultra trace analysis. Proteomic analysis is largely provided in Core B (formerly Core C) although the instrumentation in Core A is available for proteomic work. The Analytical Core provides walk-up instrumentation as well as services and research collaborations to the projects. The services include assistance with other campus facilities such as nuclear magnetic resonance (NMR). Core A advances many analytical technologies, including preparation, throughput and accuracy by developing robotic procedures, sample clean up, approaches to sample preparation and derivatization. Core A supports the development of both global and pathway selective metabolomic techniques and their applications to hypothesis generation and testing as well as for biomarker development. It carries out bioassay driven fractionation of hazardous mixtures, in collaboration with the projects and provides education for Superfund scientists in analytical chemistry. The LLNL component provides access to a variety of advanced instruments but focuses on the sub attomole (i.e. 10[-18]) sensitivity of AMS in support of projects using [14]C as a heavy isotopes. This technique for the first time allows human monitoring of metabolism of hazardous chemicals at environmentally realistic levels using exceptionally low levels of tracers. Priority targets for AMS are to quantify human metabolism of the insecticide permethrin and the personal care product triclocarban. Core A scientists provide one-on-one assistance in analytical chemistry, data generation and analysis and on going formal training in analytical techniques for Superfund researchers. Thus, Core A provides the broad analytical support necessary for developing and using biomarkers of exposure and effect.