Research Support Core: Analytical Chemistry Core

研究支持核心：分析化学核心

• 批准号: 7936582
• 负责人: BRUCE D HAMMOCK
• 金额: $ 28.51万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7936582
• 关键词: Analytical Chemistry; Biological Assay; Biological Markers; Biosensor; Chromatography; Collaborations; Computers and Advanced Instrumentation; Data; Detection; Development; Drug Kinetics; Education; Emerging Technologies; Environmental Health; Evaluation; Facility Accesses; Fractionation; Generations; Hazardous Chemicals; Hazardous Substances; Health Services Research; Human; Hybrids; Insecticides; Isotopes; Label; Laboratories; Mass Spectrum Analysis; Measurement; Metabolism; Methods; Monitor; Nuclear Magnetic Resonance; Pathway interactions; Permethrin; Preparation; Procedures; Protein Analysis; Proteomics; Quality Control; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Robotics; Sampling; Scientist; Self Care; Services; Superfund; Techniques; Technology; Testing; Time; Tracer; Training; Validation; Walking; Work; accelerator mass spectrometry; analytical method; base; chemical standard; environmental chemical; instrument; instrumentation; interest; metabolomics; programs; small molecule; theories; triclocarban

The Analytical Core is a central resource composed of two laboratories that facilitate-development and application of modern analytical methods to solve key problems encountered by the components of the Superfund Program. The first is the Superfund Analytical Laboratory which provides a range of instrument services with emphasis on mass spectrometry and chromatography and small molecule analysis. The second is the Accelerator Mass Spectrometry Facility with 2 AMS instruments at Lawrence Livermore National Laboratory for ultra trace analysis. Proteomic analysis is largely provided in Core B (formerly Core C) although the instrumentation in Core A is available for proteomic work. The Analytical Core provides walk-up instrumentation as well as services and research collaborations to the projects. The services include assistance with other campus facilities such as nuclear magnetic resonance (NMR). Core A advances many analytical technologies, including preparation, throughput and accuracy by developing robotic procedures, sample clean up, approaches to sample preparation and derivatization. Core A supports the development of both global and pathway selective metabolomic techniques and their applications to hypothesis generation and testing as well as for biomarker development. It carries out bioassay driven fractionation of hazardous mixtures, in collaboration with the projects and provides education for Superfund scientists in analytical chemistry. The LLNL component provides access to a variety of advanced instruments but focuses on the sub attomole (i.e. 10[-18]) sensitivity of AMS in support of projects using [14]C as a heavy isotopes. This technique for the first time allows human monitoring of metabolism of hazardous chemicals at environmentally realistic levels using exceptionally low levels of tracers. Priority targets for AMS are to quantify human metabolism of the insecticide permethrin and the personal care product triclocarban. Core A scientists provide one-on-one assistance in analytical chemistry, data generation and analysis and on going formal training in analytical techniques for Superfund researchers. Thus, Core A provides the broad analytical support necessary for developing and using biomarkers of exposure and effect.

分析核心是由两个实验室组成的中心资源，促进现代分析方法的开发和应用，以解决超级基金计划组成部分遇到的关键问题。第一个是超级基金分析实验室，它提供一系列仪器服务，重点是质谱、色谱和小分子分析。第二个是劳伦斯利弗莫尔国家实验室的加速器质谱设备，配有2台AMS仪器，用于超痕量分析。蛋白质组学分析主要在Core B（以前的Core C）中提供，尽管Core A中的仪器可用于蛋白质组学工作。“分析核心”为项目提供无梯仪器、服务和研究合作。这些服务包括协助使用其他校园设施，如核磁共振（NMR）。Core A通过开发机器人程序，样品清理，样品制备和衍生化方法，推进了许多分析技术，包括制备，吞吐量和准确性。Core A支持全球和途径选择性代谢组学技术的发展及其在假设生成和测试以及生物标志物开发中的应用。它与项目合作，进行生物测定驱动的有害混合物分馏，并为超级基金科学家提供分析化学方面的教育。LLNL组件提供了各种先进仪器的访问，但侧重于AMS的子原子（即10[-18]）灵敏度，以支持使用[14]C作为重同位素的项目。这项技术首次允许人类使用极低水平的示踪剂在环境现实水平上监测危险化学品的代谢。AMS的优先目标是量化杀虫剂氯菊酯和个人护理用品三氯卡班的人体代谢。核心A科学家在分析化学、数据生成和分析方面提供一对一的帮助，并为超级基金研究人员提供分析技术方面的正式培训。