STRUCTURE OF THE U2-U6 COMPLEX

U2-U6 复合体的结构

• 批准号: 8169025
• 负责人: Samuel E Butcher
• 金额: $ 3.08万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169025
• 关键词: Bacteriophage Pf1; COSY; Catalytic Domain; Complex; Computer Retrieval of Information on Scientific Projects Database; Evolution; Funding; Grant; Institution; Measures; NOESY; RNA; Research; Research Personnel; Residual state; Resources; Saccharomyces cerevisiae; Sampling; Source; Spliceosomes; Structure; Temperature; U6 small nuclear RNA; United States National Institutes of Health; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the structure of the U2-U6 snRNA complex from S. cerevisiae. This large RNA complex has been highly conserved throughout eukaryotic evolution, and forms the catalytic core of the spliceosome. The RNA structure will be investigated at a range of temperatures, pH and ionic conditions. NMR experiments that we will need to collect include: 1D (1H), 2D NOESY, 2D 1H-15N HSQC (normal and TROSY enhanced), 2D 1H-15N HNN COSY, 2D 1H-13C HSQC (normal and TROSY enhanced), 2D NOESY, 2D 1H,13C filter/edit NOESY. Residual dipolar couplings will be measured for samples in both isotropic and partially aligned states (via addition of Pf1 phage).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们正在研究S.啤酒。 这种大的RNA复合物在整个真核生物进化过程中高度保守，并形成剪接体的催化核心。 将在一定范围的温度、pH值和离子条件下研究RNA结构。 我们需要收集的NMR实验包括：1D（1H），2D NOESY，2D 1H-15 N HSQC（正常和TROSY增强），2D 1H-15 N HNN COSY，2D 1H-13 C HSQC（正常和TROSY增强），2D NOESY，2D 1H，13 C过滤/编辑NOESY。将测量各向同性和部分对齐状态下样品的残留偶极耦合（通过添加Pf 1噬菌体）。