NMR User Program at NMRFAM

NMRFAM 的 NMR 用户计划

• 批准号: 10192904
• 负责人: Samuel E Butcher
• 金额: $ 96.66万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10192904
• 关键词: Address; Adopted; Advisory Committees; Area; Automation; Biological; Biomedical Research; Biophysics; Calibration; Capital; Cell Nucleus; Communities; Complex; Computer software; Consult; Cryoelectron Microscopy; Custom; Data; Data Analyses; Data Collection; Data Security; Detection; Development; Doctor of Philosophy; Educational workshop; Emerging Technologies; Ensure; FAIR principles; Goals; Helium; Home; Human Resources; Hybrids; Individual; Infrastructure; Institution; Isotopes; Knowledge; Ligands; Liquid substance; Magic; Magnetic Resonance; Maintenance; Membrane Proteins; Methods; Mission; Molecular Weight; Monoclonal Antibody R24; NMR Spectroscopy; National Center for Research Resources; National Institute of General Medical Sciences; Natural Products; Nucleic Acids; Performance; Pharmaceutical Preparations; Phase; Physiologic pulse; Procedures; Proteins; Protocols documentation; Quality Control; Recovery; Recycling; Reproducibility; Research Support; Resource Development; Resources; Sampling; Schedule; Scientist; Services; Site; Standardization; Structure; Surveys; System; Technology; Testing; Time; Training; Tube; United States National Institutes of Health; Work; base; collaborative approach; community engagement; computerized data processing; cryogenics; cyber security; data quality; data sharing; design; experience; experimental study; improved; instrument; instrumentation; interest; knowledge base; metabolomics; next generation; operation; outreach; programs; protein structure; recruit; repaired; screening; solid state nuclear magnetic resonance; structural biology; tool; training opportunity

PROJECT SUMMARY The National Magnetic Resonance Facility at Madison (NMRFAM) provides timely and reliable access to mature NMR technology for application to a wide array of biomedical research questions. NMRFAM has staff with decades of experience in facility operation and maintenance, a dozen high-field NMR spectrometers equipped with state-of-the-art probe technology, and facility infrastructure (ample space, uninterrupted power systems, environmental control, helium recovery) second to none, and NMRFAM has been at the forefront of standardization, data stewardship through FAIR (findable, accessible, interoperable and reusable) data principles and dissemination of best practices to the community. Support through the NIGMS R24 program of NMRFAM through the R24 mechanism at NIGMS would support broad access to this exceptional resource in Madison to leverage economies of scale, including stable and efficient operation that maximizes data quality and minimizes instrument down time, facilitating many types of experiments that scientists are not able to perform at their home institutions. Furthermore, the proposed next generation user program improves the rigor and reproducibility of NMR data collection through instrument quality control, standard operating procedures, new sample standards for microcrystalline proteins and nucleic acids, membrane proteins and fibrils, including development of approaches for dynamics and function as well as structure. NMRFAM staff provide an exceptional level of service tailored to the needs of individual users and projects, serving arguably the broadest user base of any NMR facility in the US. NMRFAM actively seeks to support diverse users through outreach and user training opportunities (workshops, video tutorials, protocols) to teach users how to understand and analyze their NMR data. NMRFAM has a goal of providing users more than spectra, and the facility provides staff support and automation tools to return deliverables: i.e., scientific knowledge not only data. The NMRFAM mission lowers barriers to NMR and maximizes the impact of the facility when users can successfully apply the NMR data to answer their biological question of interest. NMRFAM has built a reputation as a top-level facility for external users and this project will maintain affordable access to NMRFAM. NMRFAM staff will regularly evaluate performance criteria and consult with the external advisory committee and facility users so that the facility continues to remain at the cutting edge and meet the needs of its user base.

项目摘要 位于麦迪逊的国家磁共振设施（NMRFAM）提供了及时可靠的访问成熟的 核磁共振技术应用于广泛的生物医学研究问题。全国小额贷款和家庭救济委员会的工作人员 数十年的设备操作和维护经验，配备了十几台高场NMR光谱仪， 具有最先进的探针技术和设施基础设施（充足的空间，不间断的电力系统， 环境控制，氦气回收）首屈一指，NMRFAM一直处于最前沿， 标准化，通过FAIR（可查找、可访问、可互操作和可重复使用）数据进行数据管理 原则和向社区传播最佳做法。通过NIGMS R24计划提供支持， 通过NIGMS的R24机制，NMRFAM将支持广泛获得这一特殊资源， 麦迪逊利用规模经济，包括稳定和高效的运营，最大限度地提高数据质量， 最大限度地减少仪器停机时间，方便科学家无法在 他们的家乡机构。此外，提出的下一代用户程序提高了严格性， 通过仪器质量控制、标准操作程序、新的 微晶蛋白质和核酸、膜蛋白和原纤维的样品标准品，包括 发展动力学和功能以及结构的方法。全国小额贷款和家庭援助组织工作人员提供 根据个人用户和项目的需求量身定制的卓越服务水平，可以说是最广泛的 美国任何核磁共振设备的用户群。NMRFAM积极寻求通过外联支持各种用户 和用户培训机会（讲习班、视频教程、协议），以教用户如何理解和 分析核磁共振数据NMRFAM的目标是为用户提供比光谱更多的信息，该设施提供 工作人员支持和自动化工具，以返回可交付成果：即，科学知识不仅仅是数据。国家医疗和家庭福利部 使命降低了核磁共振的障碍，并在用户能够成功应用 核磁共振数据来回答他们感兴趣的生物学问题。NMRFAM已经建立了作为顶级设施的声誉 这一项目将保持以负担得起的价格获得国家小额信贷和小额金融服务机构。NMRFAM工作人员将定期 评估绩效标准，并与外部咨询委员会和设施用户协商， 该设施继续保持在最前沿，并满足其用户群的需求。