HIV FRAMESHIFT SITE RNA LIGAND INTERACTIONS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 HIV-1移码位点RNA是一种在翻译过程中刺激程序性-1核糖体移码的RNA结构。 这种移码对于酶蛋白逆转录酶、整合酶和蛋白酶的表达是必需的。 因此，这种RNA结构对于病毒复制是必需的。 我们已经进行了高通量筛选，以寻找与这种RNA结构结合的小的药物样分子。 从该筛选中，我们已经鉴定出202个阳性命中物作为潜在的RNA结合配体。 要求NMR时间来研究这些小分子与HIV-1移码位点RNA的相互作用，以及RNA结构如何受到配体结合的影响。 要进行的实验包括1D WATERLOGSY、2D NOESY、2D 1H-15 N HSQC和2D 1H-13 C HSQC。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The HIV-1 frameshift site RNA is an RNA structure that stimulates a programmed -1 ribosomal frameshift during translation. This frameshift is required for expression of the enzymatic proteins reverse transcriptase, integrase and protease. Thus this RNA structure is essential for viral replication. We have performed a high-throughput screen to search for small, drug-like molecules that bind to this RNA structure. From this screen, we have identified 202 positive hits as potential RNA-binding ligands. NMR time is requested to investigate the interaction of these small molelcules with the HIV-1 frameshift site RNA, and in turn how the RNA structure is affected by ligand binding. Experiments to be performed include 1D WATERLOGSY, 2D NOESY, 2D 1H-15N HSQC and 2D 1H-13C HSQC.