U6 RNA - PRP24 COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 需要核磁共振时间来研究U6 RNA结合蛋白Prp24的结构。经2D HSQC测定，该蛋白大小为25 kDa，折叠良好。我们打算解决Prp24的结构问题，并为此制作了13C，15N双标记核磁共振样品。作业将通过Eghbalnia等人最近开发的自动作业程序开心果获得。我们将收集蛋白质共振指定的标准三重共振实验套件(HNCA、HNCACB、CBCACONH、3D HCCH TOCSY、3D NOESY-[1H，15N]-HSQC和3D NOESY-[1H，13C]-HSQC)。这些实验将在不同的RNA和蛋白质结构上进行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NMR time is requested to investigate the structure of a U6 RNA binding protein, Prp24. This protein is 25 kDa and well folded as determined by 2D HSQC. We intend to solve the structure of Prp24, and have produced a 13C, 15N double labeled NMR sample for this purpose. Assignments will be obtained through an automated assignment procedure, PISTACHIO, recently developed by Eghbalnia et al. We will collect the standard suite of triple resonance experiments for protein resonance assignments (HNCA, HNCACB, CBCACONH, 3D HCCH TOCSY,3D NOESY-[1H,15N]-HSQC, and 3D NOESY-[1H,13C]-HSQC. These experiments will be performed on various constructs of RNA and protein.