U6 RNA - PRP24 COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 要求NMR时间来研究U6 RNA结合蛋白Prp 24的结构。 该蛋白质为25 kDa，通过2D HSQC确定折叠良好。 我们打算解决Prp 24的结构，并已为此目的制备了13 C，15 N双标记的NMR样品。 将通过Eghbalnia等人最近开发的自动分配程序PISTACHIO获得蛋白质。我们将收集用于蛋白质共振分配的标准三重共振实验套件（HNCA、HNCACB、CBCACONH、3D HCCH TOCSY、3D NOESY-[1H，15 N]-HSQC和3D NOESY-[1H，13 C]-HSQC。 这些实验将在RNA和蛋白质的各种构建体上进行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NMR time is requested to investigate the structure of a U6 RNA binding protein, Prp24. This protein is 25 kDa and well folded as determined by 2D HSQC. We intend to solve the structure of Prp24, and have produced a 13C, 15N double labeled NMR sample for this purpose. Assignments will be obtained through an automated assignment procedure, PISTACHIO, recently developed by Eghbalnia et al. We will collect the standard suite of triple resonance experiments for protein resonance assignments (HNCA, HNCACB, CBCACONH, 3D HCCH TOCSY,3D NOESY-[1H,15N]-HSQC, and 3D NOESY-[1H,13C]-HSQC. These experiments will be performed on various constructs of RNA and protein.