TETRALOOP RECEPTOR RNA STRUCTURAL INVESTIGATION

四环受体 RNA 结构研究

• 批准号: 8361194
• 负责人: Samuel E Butcher
• 金额: $ 1.36万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361194
• 关键词: Bacteriophage Pf1; Binding; COSY; Functional RNA; Funding; Grant; Investigation; Kinetics; Measures; Mediating; NOESY; National Center for Research Resources; Principal Investigator; RNA; Research; Research Infrastructure; Residual state; Resources; Sampling; Source; Structure; TOCSY; Temperature; United States National Institutes of Health; Work; cost; receptor; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are investigating the structure of the free tetraloop receptor. The tetraloop receptor is a commonly occurring motif that mediates tertiary folding of large, non-coding RNAs. We are investigating the tetraloop receptor structure as a function of temperature and ionic conditions. Previous work shows that the tetraloop receptor undergoes a conformational change upon tetraloop binding. We are also investigating the kinetics of this conformational change by NMR. NMR experiments that we will need to collect include: 1D (1H), 2D NOESY, 2D 1H-15N HSQC (normal and TROSY enhanced), 2D 1H-15N HNN COSY, 2D 1H-13C HSQC (normal and TROSY enhanced), 2D NOESY, 2D 1H,13C filter/edit NOESY, 3D 1H(13C)1H NOESY-HSQC, 3D 1H(13C)1H HCCH TOCSY and 3D 1H(13C)1H HCCH COSY. Residual dipolar couplings will be measured for samples in both isotropic and partially aligned states (via addition of Pf1 phage).

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们正在研究游离四环受体的结构。 四环受体是一种常见的基序，介导大的非编码RNA的三级折叠。 我们正在研究的四环受体结构作为温度和离子条件的函数。 以前的工作表明，四环受体经历了一个构象变化后，四环结合。 我们也正在研究这种构象变化的动力学NMR。 我们需要收集的NMR实验包括：1D（1H），2D NOESY，2D 1H-15 N HSQC（正常和TROSY增强），2D 1H-15 N HNN COSY，2D 1H-13 C HSQC（正常和TROSY增强），2D NOESY，2D 1H，13 C过滤/编辑NOESY，3D 1H（13 C）1H NOESY-HSQC，3D 1H（13 C）1H HCCH TOCSY和3D 1H（13 C）1H HCCH COSY。将测量各向同性和部分对齐状态下样品的残留偶极耦合（通过添加Pf 1噬菌体）。