STRUCTURE DETERMINATION OF RNA IN SUPERCOOLED WATER

过冷水中 RNA 的结构测定

• 批准号: 8169015
• 负责人: Samuel E Butcher
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169015
• 关键词: Area; Base Pairing; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Hydroxyl Radical; Institution; NMR Spectroscopy; Nucleic Acids; Protons; RNA; Research; Research Personnel; Resources; Sampling; Source; Structure; Techniques; Temperature; United States National Institutes of Health; Water; interest; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. : High quality structure determination of RNA molecules using NMR spectroscopy became a wire area of interest in recent past. Tertiary structure of RNA relay on the base pair formation between the nucleic acids. The labile protons those are involving in the base pair formation are exchange with water at ambient temperatures. We propose to determine the tertiary structure of RNA at sub zero possibly at -17C temperatures using the supercooled water techniques. At that temperature we possibly observe the exchangeable protons such as imino, sugar hydroxyls that will help giving more conformational distance restrains to derive high quality RNA structure. We selected U6ISL RNA to study the supercooled water. For this study we are going to use the RT probe to reach the sample temperature to -17C.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 利用核磁共振波谱法对RNA分子进行高质量的结构测定是近年来研究的热点。RNA的三级结构依赖于核酸之间碱基对的形成。参与碱基对形成的不稳定质子在环境温度下与水交换。我们建议使用过冷水技术在零度以下可能在-17 ℃的温度下测定RNA的三级结构。在该温度下，我们可能会观察到可交换的质子，例如亚氨基、糖羟基，这将有助于提供更多的构象距离限制，以获得高质量的RNA结构。我们选择U6 ISL RNA来研究过冷水。在本研究中，我们将使用RT探针将样品温度达到-17 ℃。