LARGE SCALE DATA COLLECTION ON POLIOVIRUS HEAT INACTIVATED EMPTY CAPSIDS

脊髓灰质炎病毒热灭活空衣壳的大规模数据收集

• 批准号: 8169695
• 负责人: JAMES HOGLE
• 金额: $ 2.33万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169695
• 关键词: Capsid; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Data Collection; Funding; Genome; Grant; Heating; Human poliovirus; In Vitro; Institution; Liposomes; Membrane; Membrane Fusion; Modeling; Nucleoproteins; Pathway interactions; Polioviruses; Process; Research; Research Personnel; Resources; Site; Source; Staging; United States National Institutes of Health; Virus; in vivo; receptor; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are attempting to structurally characterize intermediates in the cell entry pathway of poliovirus, including soluble forms of the intermediates in vitro, membrane associated forms of the intermediates in vitro using a receptor-decorated liposome model, and intracellular forms of the intermediates in vivo. Although membrane fusion provides a conceptually simple pathway for genome delivery in enveloped viruses, nonenveloped viruses must provide a mechanism for transporting the genome or nucleoprotein complexes containing the genome across a membrane. This process is poorly understood. This specific experiment aims to characterize intermediates in various stages of releasing the genome, in hope of determining the site and mechanism of externalization.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们正在尝试在结构上表征脊髓灰质炎病毒细胞进入途径中的中间体，包括体外中间体的可溶性形式，使用受体饰面的脂质体模型和中间体的细胞内中间体的体外形式的中间体的膜相关形式。 尽管膜融合为包围病毒中的基因组传递提供了一种简单的途径，但非发育的病毒必须提供一种机制来运输基因组或核蛋白复合物，这些基因组或核蛋白复合物在膜上含有基因组。 这个过程对此知之甚少。 该特定的实验旨在表征释放基因组的各个阶段中间人，以期确定外部化的位点和机制。