LARGE SCALE DATA COLLECTION ON POLIOVIRUS HEAT INACTIVATED EMPTY CAPSIDS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们正试图从结构上表征脊髓灰质炎病毒进入细胞途径中的中间体，包括体外可溶形式的中间体，使用受体修饰的脂质体模型体外膜相关形式的中间体，以及体内细胞内形式的中间体。 尽管膜融合为包膜病毒中的基因组递送提供了概念上简单的途径，但无包膜病毒必须提供一种转运基因组或含有基因组的核蛋白复合物穿过膜的机制。 人们对这一过程知之甚少。 这个特定的实验旨在表征释放基因组的各个阶段的中间体，希望确定外部化的位点和机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are attempting to structurally characterize intermediates in the cell entry pathway of poliovirus, including soluble forms of the intermediates in vitro, membrane associated forms of the intermediates in vitro using a receptor-decorated liposome model, and intracellular forms of the intermediates in vivo. Although membrane fusion provides a conceptually simple pathway for genome delivery in enveloped viruses, nonenveloped viruses must provide a mechanism for transporting the genome or nucleoprotein complexes containing the genome across a membrane. This process is poorly understood. This specific experiment aims to characterize intermediates in various stages of releasing the genome, in hope of determining the site and mechanism of externalization.