LARGE SCALE DATA COLLECTION ON POLIOVIRUS HEAT INACTIVATED EMPTY CAPSIDS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们正试图从结构上表征细胞进入途径中的中间产物 脊髓灰质炎病毒，包括体外可溶中间体、膜相关形式 使用受体修饰的脂质体模型在体外和细胞内对中间体进行研究 体内中间体的形态。 尽管膜融合在概念上为基因组传递提供了一种简单的途径 包膜病毒，非包膜病毒必须提供一种机制来传输 跨膜包含基因组的基因组或核蛋白复合体。这 人们对这一过程知之甚少。这项特定的实验旨在表征 释放基因组的各个阶段，希望确定基因的位置和机制 外部化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are attempting to structurally characterize intermediates in the cell entry pathway of poliovirus, including soluble forms of the intermediates in vitro, membrane associated forms of the intermediates in vitro using a receptor-decorated liposome model, and intracellular forms of the intermediates in vivo. Although membrane fusion provides a conceptually simple pathway for genome delivery in enveloped viruses, nonenveloped viruses must provide a mechanism for transporting the genome or nucleoprotein complexes containing the genome across a membrane. This process is poorly understood. This specific experiment aims to characterize intermediates in various stages of releasing the genome, in hope of determining the site and mechanism of externalization.