PROTEOMICS ANALYSIS OF SCHISTOSOME HOST-INVASION AND METABOLISM

血吸虫宿主入侵和代谢的蛋白质组学分析

• 批准号: 8169748
• 负责人: James H. McKerrow
• 金额: $ 7.07万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169748
• 关键词: Classification; Computer Retrieval of Information on Scientific Projects Database; Drug Delivery Systems; Exposure to; Funding; Grant; Human; Immune; Incubated; Institution; Larva; Metabolism; Methods; Peptide Hydrolases; Proteins; Proteomics; Research; Research Personnel; Resources; Schistosoma; Schistosoma japonicum; Schistosoma mansoni; Schistosomiasis; Skin; Source; United States National Institutes of Health; Work; secretory protein; vaccine candidate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is focused on understanding the interaction between human and schistosoma proteins, with the aim of identifying drug targets and/or vaccine candidates for protection from schistosomiasis. Previous work on this project has been to analyze active secretory proteins from Schistosoma mansoni during initial host exposure to invasive larvae as a method for identifying functional proteins involved in invasion and host immune evasion. This work has expanded to analyze the the natural substrates for the proteases of invasive schistosome larvae in skin, where Schistosome cercariae are incubated with sections of human skin and products of degradation extracted. In a parallel track, the active proteases across multiple species including Schistosoma mansoni, Schistosoma japonicum and Schistosomatium douthitti were characterized for proteolytic classification and activity.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的重点是了解人类和血吸虫蛋白之间的相互作用，目的是确定保护血吸虫病的药物靶点和/或候选疫苗。 该项目以前的工作一直是分析曼氏血吸虫在初始宿主暴露于入侵幼虫期间的活性分泌蛋白，作为鉴定参与入侵和宿主免疫逃避的功能蛋白的方法。这项工作已经扩展到分析皮肤中侵入性血吸虫幼虫蛋白酶的天然底物，其中血吸虫尾蚴与人类皮肤切片一起孵育，并提取降解产物。 在一个平行的轨道上，活性蛋白酶跨多个物种，包括血吸虫曼氏血吸虫，日本血吸虫和杜氏血吸虫的蛋白水解分类和活性的特点。