GIARDIA LAMBLIA CYSTEINE PROTEASES: TRAFFICKING, LOCALIZATION, AND FUNCTION

贾第鞭毛虫半胱氨酸蛋白酶:运输、定位和功能

基本信息

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Giardia is an important model for cell biology because it represents the most basal of eukaryotic lineages based on sequence analysis of conserved genes. Giardia trophozoites lack many typical eukaryotic organelles such as mitochondria, peroxisomes, and protein trafficking compartments such as a classical Golgi apparatus and secretory granules. Instead, Giardia employs a simple endomembrane system to export proteins to distinct intracellular locations. We have discovered a novel protein compartment in Giardia that appears to serve as ER and endosome. Using reporter-constructs, confocal microscopy, and ultrastructural analysis, we have shown that Giardia cysteine proteases localize to a tubulovesicular network with an ER-like structure peripheral to the perinuclear membrane. Labeled proteins are rapidly endocytosed into this compartment and degraded by these Giardia cysteine proteases. It therefore appears that Giardia contains a transitional endomembranous structure that may pre-date compartmentalization of endocytic/lysosomal functions and the endoplasmic reticulum. Giardia cysteine proteases may function to break down endocytosed nutrients. However, it remains unclear which members of the Giardia cysteine protease family are responsible for this activity. Cysteine protease activity can be isolated and purified from Giardia lysate using FPLC. This lysate fraction can then be submitted for mass spectrometry analysis to determine the gene products responsible for this novel activity.
这个子项目是利用这些资源的众多研究子项目之一

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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James H. McKerrow其他文献

Binding Mode and Potency of N-Indolyloxopyridinyl-4-aminopropanyl-Based Inhibitors Targeting Trypanosoma cruzi CYP51
N-吲哚氧吡啶基-4-氨基丙基抑制剂针对克氏锥虫 CYP51 的结合模式和效力
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    7.3
  • 作者:
    D. F. Vieira;Jun Yong Choi;C. M. Calvet;J. Siqueira;Jonathan B. Johnston;Danielle Kellar;Jiri Gut;Michael D. Cameron;James H. McKerrow;William R. Roush;L. Podust
  • 通讯作者:
    L. Podust
Elastases and elastin degradation.
弹性蛋白酶和弹性蛋白降解。
Giardia lamblia cysteine proteases
  • DOI:
    10.1007/s00436-006-0149-4
  • 发表时间:
    2006-04-06
  • 期刊:
  • 影响因子:
    2.000
  • 作者:
    Kelly N. DuBois;Marla Abodeely;Mohammed Sajid;Juan C. Engel;James H. McKerrow
  • 通讯作者:
    James H. McKerrow
Proteinase production by the parasitic cycle of the pathogenic fungus Coccidioides immitis
病原真菌粗球孢子菌寄生循环产生的蛋白酶
  • DOI:
  • 发表时间:
    1987
  • 期刊:
  • 影响因子:
    3.1
  • 作者:
    Steven Resnick;Demosthenes Pappagianis;James H. McKerrow
  • 通讯作者:
    James H. McKerrow
Cloning of a cysteine protease required for the molting of <em>Onchocerca volvulus</em> third stage larvae.
  • DOI:
    10.1016/s0021-9258(19)67340-0
  • 发表时间:
    1997-02-14
  • 期刊:
  • 影响因子:
  • 作者:
    Sara Lustigman;James H. McKerrow;Kashmira Shah;Jing Lui;Tellervo Huima;Matt Hough;Betsy Brotman
  • 通讯作者:
    Betsy Brotman

James H. McKerrow的其他文献

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{{ truncateString('James H. McKerrow', 18)}}的其他基金

Evaluation of a cathepsin S inhibitor as a potential drug for Chagas disease
组织蛋白酶 S 抑制剂作为恰加斯病潜在药物的评价
  • 批准号:
    8996043
  • 财政年份:
    2015
  • 资助金额:
    $ 0.01万
  • 项目类别:
HYDROLYSIS OF HEMOGLOBIN BY SCHISTSOMA MANSONI CATHEPSIN B-LIKE CYSTEINEPROTEASE
曼氏血吸虫组织蛋白酶 B 样半胱氨酸蛋白酶水解血红蛋白
  • 批准号:
    8363752
  • 财政年份:
    2011
  • 资助金额:
    $ 0.01万
  • 项目类别:
STRUCTURE BASED DRUG DESIGN FOR TREATMENT OF PARASITIC AND VIRAL DISEASES
用于治疗寄生虫和病毒性疾病的基于结构的药物设计
  • 批准号:
    8363751
  • 财政年份:
    2011
  • 资助金额:
    $ 0.01万
  • 项目类别:
PROTEOMICS ANALYSIS OF SCHISTOSOME HOST-INVASION AND METABOLISM
血吸虫宿主入侵和代谢的蛋白质组学分析
  • 批准号:
    8363754
  • 财政年份:
    2011
  • 资助金额:
    $ 0.01万
  • 项目类别:
MECHANISM OF HOST INVASION BY SCHISTOSOMES
血吸虫宿主入侵机制
  • 批准号:
    8363596
  • 财政年份:
    2011
  • 资助金额:
    $ 0.01万
  • 项目类别:
GIARDIA LAMBLIA CYSTEINE PROTEASES: TRAFFICKING, LOCALIZATION, AND FUNCTION
贾第鞭毛虫半胱氨酸蛋白酶:运输、定位和功能
  • 批准号:
    8169751
  • 财政年份:
    2010
  • 资助金额:
    $ 0.01万
  • 项目类别:
HYDROLYSIS OF HEMOGLOBIN BY SCHISTSOMA MANSONI CATHEPSIN B-LIKE CYSTEINEPROTEASE
曼氏血吸虫组织蛋白酶 B 样半胱氨酸蛋白酶水解血红蛋白
  • 批准号:
    8169746
  • 财政年份:
    2010
  • 资助金额:
    $ 0.01万
  • 项目类别:
STRUCTURE BASED DRUG DESIGN FOR TREATMENT OF PARASITIC AND VIRAL DISEASES
用于治疗寄生虫和病毒性疾病的基于结构的药物设计
  • 批准号:
    8169745
  • 财政年份:
    2010
  • 资助金额:
    $ 0.01万
  • 项目类别:
Lead Identification to Clinical Candidate Selection: Drugs for Chagas Disease
临床候选药物选择的线索识别:恰加斯病药物
  • 批准号:
    8107529
  • 财政年份:
    2010
  • 资助金额:
    $ 0.01万
  • 项目类别:
Lead Identification to Clinical Candidate Selection: Drugs for Chagas Disease
临床候选药物选择的线索识别:恰加斯病药物
  • 批准号:
    8499225
  • 财政年份:
    2010
  • 资助金额:
    $ 0.01万
  • 项目类别:

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