PROTEOMICS ANALYSIS OF SCHISTOSOME HOST-INVASION AND METABOLISM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的重点是了解人类和血吸虫蛋白之间的相互作用，目的是确定预防血吸虫病的药物靶标和/或候选疫苗。该项目之前的工作是分析曼氏血吸虫最初接触入侵幼虫时的活性分泌蛋白，以此作为识别参与入侵和宿主免疫逃避的功能蛋白的方法。这项工作已扩展到分析侵袭性血吸虫幼虫皮肤中蛋白酶的天然底物，血吸虫尾虫与人体皮肤切片和提取的降解产物孵育。在平行的轨道上，对包括曼氏血吸虫、日本血吸虫和杜氏血吸虫在内的多个物种的活性蛋白水解酶进行了蛋白分解分类和活性鉴定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is focused on understanding the interaction between human and schistosoma proteins, with the aim of identifying drug targets and/or vaccine candidates for protection from schistosomiasis. Previous work on this project has been to analyze active secretory proteins from Schistosoma mansoni during initial host exposure to invasive larvae as a method for identifying functional proteins involved in invasion and host immune evasion. This work has expanded to analyze the the natural substrates for the proteases of invasive schistosome larvae in skin, where Schistosome cercariae are incubated with sections of human skin and products of degradation extracted. In a parallel track, the active proteases across multiple species including Schistosoma mansoni, Schistosoma japonicum and Schistosomatium douthitti were characterized for proteolytic classification and activity.